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During the biotransformation of MMF to MPA, additional metabolites including MPA phenol glucuronide (MPAG), MPA acyl glucuronide (AcMPAG) and MPA phenol glucoside (MPG) are formed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To define the noncompartmental pharmacokinetic (PK) parameters of three single doses of intravenous (i.v.) MMF and its downstream metabolites in healthy horses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Six healthy Standardbred mares.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Generic MMF (Par Pharmaceuticals; Chestnut Ridge, NY, USA) was reconstituted and administered as a single i.v. bolus at 1.0\u2009mg\/kg, 5.0\u2009mg\/kg and 10.0\u2009mg\/kg with an eight day washout between treatments. Blood samples were collected immediately before MMF administration and over 24\u2009h. A liquid chromatography\u2013tandem mass spectrometry assay was developed following FDA guidance to determine plasma MMF, MPA, MPAG, AcMPAG and MPG concentrations. Plasma concentrations were analysed independently, followed by calculation of geometric mean and coefficient of variation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Noncompartmental PK parameters were determined for MMF and all metabolites at all doses. MMF was rapidly converted to MPA in all horses. Each incremental dose of MMF resulted in increases in C<jats:sub>max<\/jats:sub>and AUC<jats:sub>inf<\/jats:sub>_obs for MPA and the three additional metabolites. Within the 10\u2010fold dose range, the increase in C<jats:sub>max<\/jats:sub>and AUC<jats:sub>inf<\/jats:sub>_obs for MMF and its metabolites was nonlinear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Horses biotransform MMF into MPA, MPAG, AcMPAG and MPG via the glucuronidation and glucosidation clearance pathways. Equine reference PK profiles for MPA and the metabolites, MPAG, AcMPAG and MPG were established.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13109","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T10:34:37Z","timestamp":1659695677000},"page":"222-234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Noncompartmental pharmacokinetics of three intravenous mycophenolate mofetil concentrations in healthy Standardbred mares"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6305-9307","authenticated-orcid":false,"given":"Dylan L.","family":"Burroughs","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine, The Ohio State University Columbus OH 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