{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:36:28Z","timestamp":1771475788116,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T00:00:00Z","timestamp":1661299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1F1A1055786"],"award-info":[{"award-number":["2021R1F1A1055786"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hair cycle arrest (HCA) is a noninflammatory alopecic disease affecting various dog breeds, particularly Pomeranian dogs. This disease is probably a hereditary disorder considering the strong breed predisposition. Despite efforts to identify the pathogenesis of this disease, an underlying specific cause is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify candidate gene mutations for HCA in Pomeranian dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Four Pomeranian dogs diagnosed with HCA and four unaffected Pomeranian dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Whole blood was used for DNA extraction. Whole\u2010genome sequencing (WGS) was performed, and variants were analysed using a Genome Analysis Toolkit (GATK) and SnpEff. All reads were aligned to the reference genome, Dog10K_Boxer_Tasha. Sanger sequencing was performed to define the complex mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 113 variants of mitochondrial DNA were found to be effective gene mutations in the eight dogs. The affected dogs showed significantly increased effective mutations (average 57 variants) compared with unaffected dogs (average eight variants; <jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05). There was no significant difference in the number of chromosomal DNA mutations between the two groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Importance<\/jats:title><jats:p>We suggest that an increased number of mitochondrial gene mutations are features for HCA in Pomeranian dogs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13114","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T11:19:08Z","timestamp":1661339948000},"page":"545-552","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The number of mitochondrial <scp>DNA<\/scp> mutations as a genetic feature for hair cycle arrest (alopecia X) in Pomeranian dogs"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9524-024X","authenticated-orcid":false,"given":"Yeong\u2010Hun","family":"Kang","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science, College of Veterinary Medicine Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1157-2237","authenticated-orcid":false,"given":"Jae\u2010Eun","family":"Hyun","sequence":"additional","affiliation":[{"name":"Institute of Animal Medicine, College of Veterinary Medicine Gyeongsang National University  Jinju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7113-0361","authenticated-orcid":false,"given":"Cheol\u2010Yong","family":"Hwang","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science, College of Veterinary Medicine Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,24]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"1317","article-title":"Identifying and treating sex\u2010hormone dermatoses in dogs","volume":"85","author":"Chalmers S","year":"1990","journal-title":"Vet Med"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2004.00403.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2004.00372.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12236"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10495390701547461"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.163.4.121"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0186469"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-017-1280-y"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(02)00070-X"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2013.12.005"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2012.08.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13394"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16242"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.13971"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.107524.110"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2001.218.729"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.025643"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2009.09.005"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmn021"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4085"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14536"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0765-9"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004670"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.42.110807.091442"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.030866"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a011254"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111994200"},{"key":"e_1_2_10_30_1","unstructured":"Da Costa AntasPR.Identification and characterisation of novel wnt regulators in colorectal cancer;2017PhD thesis University College London London. 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