{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T20:51:16Z","timestamp":1770670276655,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There is a need for alternative topical therapies as a consequence of the increased prevalence of meticillin\u2010resistant<jats:italic>Staphylococcus pseudintermedius<\/jats:italic>(MRSP) skin infections in dogs. Sodium oxychlorosene has been used as a topical antibacterial agent in human medicine since 1955.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine whether 0.2% and 0.4% sodium oxychlorosene solutions have a bactericidal effect (&gt;3\u2010log reduction) on MRSP strains isolated from canine skin infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>A genetically heterogeneous collection of MRSP isolates from dogs was assembled from laboratories across the United States. Time\u2013kill assays were performed with 0.2% and 0.4% sodium oxychlorosene on a 0.5 McFarland standard [approximately 10<jats:sup>8<\/jats:sup>colony\u2010forming units (cfu\/ml)] suspension of each strain. The average bacterial counts (cfu\/ml) of each MRSP strain then were determined at 5, 10, 20 and 60\u2009s after exposure to sodium oxychlorosene; cfu\/ml data were converted to log<jats:sub>10<\/jats:sub>scale to calculate microbial reduction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average bacterial counts following exposure to the 0.2% solution at 5, 10, 20 and 60\u2009s were 6.94\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>, 5.63\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>, 2.96\u2009\u00d7\u200910<jats:sup>2<\/jats:sup>and 1.48\u2009\u00d7\u200910<jats:sup>2<\/jats:sup>\u00a0cfu\/ml, respectively. For the 0.4% solution, the average bacterial count at 5\u00a0s was 2.12\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u00a0cfu\/ml. No bacterial growth was observed for any MRSP strain by 10\u00a0s. The greatest reduction in cfu\/ml occurred within 5\u00a0s following exposure to each solution 3.4\u2010log and 4.9\u2010log reduction for 0.2% and 0.4%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>0.2% and 0.4% sodium oxychlorosene solutions have a bactericidal effect (&gt;99.9% reduction) against<jats:styled-content style=\"fixed-case\">MRSP<\/jats:styled-content>in vitro. Further in vivo studies are necessary to determine whether it is an appropriate alternative therapy for canine pyoderma<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13121","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T07:00:17Z","timestamp":1663052417000},"page":"33-39","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In vitro efficacy of 0.2% and 0.4% sodium oxychlorosene against meticillin\u2010resistant<i>Staphylococcus pseudintermedius<\/i>"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8052-3391","authenticated-orcid":false,"given":"Katlyn M.","family":"Lake","sequence":"first","affiliation":[{"name":"Animal Dermatology Clinic Ontario California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley C.","family":"Rankin","sequence":"additional","affiliation":[{"name":"Department of Pathobiology University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne S.","family":"Rosenkrantz","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic Tustin California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshmi","family":"Sastry","sequence":"additional","affiliation":[{"name":"Department of Pathobiology University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine North Carolina State University Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dubra Diaz","family":"Campos","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine The Ohio State University Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelli","family":"Maddock","sequence":"additional","affiliation":[{"name":"AES Veterinary Diagnostic Services North Dakota State University Fargo North Dakota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2681-0082","authenticated-orcid":false,"given":"Stephen D.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Pathobiology University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01034.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12318"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12444"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12382"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12292"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12854"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12118"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2011.01030.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.03.026"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci8010011"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268815001545"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01599"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq078"},{"key":"e_1_2_9_15_1","first-page":"613","article-title":"Prevalence of methicillin\u2010resistant Staphylococcus pseudintermedius on hand\u2010contact and animal\u2010contact surfaces in companion animal community hospitals","volume":"61","author":"Perkins AV","year":"2020","journal-title":"Can Vet J"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.07.021"},{"key":"e_1_2_9_17_1","first-page":"104","article-title":"Action of Clorpactin WCS\u201090 in microbiology. 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