{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:44:45Z","timestamp":1785797085707,"version":"3.56.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Atopic dogs often are managed with allergen\u2010specific immunotherapy (AIT) and concurrent dosages of ciclosporin (CSA) or oclacitinib to alleviate their clinical signs. Both drugs might affect proper tolerance induction by inhibiting regulatory T\u2010cell (Treg) induction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>We evaluated Treg cell numbers and serum interleukin (IL)\u201010 and transforming growth factor\u2010beta (TGF\u2010\u03b2)1 levels in dogs diagnosed with atopic dermatitis (AD) and successfully treated with either CSA or oclacitinib for nine or more months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>We included 15 dogs receiving oclacitinib, 14 dogs treated with CSA, 15 healthy dogs, 13 dogs with untreated moderate\u2010to\u2010severe AD and 15 atopic dogs controlled with AIT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Peripheral blood CD4+CD25+FOXP3+ T\u2010cell percentages were determined using flow cytometry. Serum concentrations of IL\u201010 and TGF\u2010\u03b21 were measured by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The percentage of Treg cells in the CSA group was significantly lower in comparison with the healthy group (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0003), the nontreated AD group (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0056) or the AIT group (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0186). There was no significant difference in Treg cell percentages between the CSA and oclacitinib groups, nor between the oclacitinib and the healthy, nontreated AD or AIT\u2010treated dogs. No significant differences were detected in IL\u201010 and TGF\u2010\u03b21 serum concentrations between the five groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Lower Treg cell percentages in the CSA\u2010treated dogs suggest an impact of this drug on this cell population; however, it does not necessarily mean that it diminishes tolerance. Functionality and cytokine production may be more important than the number of Treg cells. Further studies evaluating the treatment outcome of dogs receiving AIT and concurrent drugs are needed to show clinical relevance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13140","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T07:11:45Z","timestamp":1670569905000},"page":"107-114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Long\u2010term effects of ciclosporin and oclacitinib on mediators of tolerance, regulatory T\u2010cells, <scp>IL<\/scp>\u201010 and <scp>TGF<\/scp>\u2010\u03b2, in dogs with atopic dermatitis"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2772-9144","authenticated-orcid":false,"given":"Ina","family":"Herrmann","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa B.","family":"Mamo","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9993-7407","authenticated-orcid":false,"given":"Javid P.","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Flow Cytometry &amp; Cell Sorting Core, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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