{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:16:12Z","timestamp":1761664572052,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T00:00:00Z","timestamp":1684195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Grass leaf has been suspected of causing immunoglobulin (Ig)E\u2010mediated immediate hypersensitivity reactions in humans and dogs. However, most studies in this area are case\u2010control studies without in vitro data showing the involvement of IgE in the reaction. Laboratory studies have demonstrated the reactivity to a 50\u201355\u2009kDa protein with clinical signs immediately after contact with grass leaf material. The clinical findings of dogs with atopic\u2010like dermatitis immediately after contact with grass leaf material suggest the involvement of grass leaves as the allergen source.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study was designed to test the IgE\u2010reactivity of grass leaf proteins in dogs with clinical signs and positive scratch test results against grass leaf material.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The serum of 41 patients with a history of allergy and suspected to grass leaf material was immunoblotted against grass leaf extracts from five suspected grass species. The IgE\u2010positive blots were separated with 2D gel electrophoresis and analysed with mass spectrometry (MS). Commercially supplied proteins were used to validate immunoblot activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The serum of 25 dogs diagnosed with grass dermatitis had positive IgE\u2010specific immunoblot against one or more grass leaf extracts. The MS data indicated a reactive band at 55\u2009kDa to be beta\u2010amylase or RuBisCO (ribulose\u20101,5\u2010bisphosphate carboxylase\/oxygenase) large subunit (RbLS). All tested dog sera showed IgE\u2010reactivity with beta\u2010amylase and some with RbLS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Canines with clinical signs of grass\u2010related dermatitis had IgE\u2010reactivity against grass leaf proteins. Serum IgE\u2010reactivity to beta\u2010amylase and RuBisCO large subunit may indicate that these proteins act as allergens, possibly causing pruritus and skin lesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13166","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T08:35:31Z","timestamp":1684226131000},"page":"393-403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunoglobulin E\u2010specific allergens against leaf in serum of dogs with clinical features of grass leaf allergy"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7623-1898","authenticated-orcid":false,"given":"Ken","family":"Mason","sequence":"first","affiliation":[{"name":"Animal Allergy and Dermatology Service  Slacks Creek Queensland Australia"},{"name":"School of Veterinary Science University of Queensland  Gatton Queensland 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