{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:24Z","timestamp":1747188444834,"version":"3.40.5"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,7,23]],"date-time":"2023-07-23T00:00:00Z","timestamp":1690070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["pericles.pericles-gcp.literatumonline.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hymenoptera envenomation with honey bee (<jats:italic>Apis mellifera<\/jats:italic>) and paper wasp (<jats:italic>Polistes<\/jats:italic> spp.) may cause life\u2010threatening anaphylaxis in dogs. In human patients, clinical history, intradermal testing (IDT) and measurement of allergen\u2010specific serological immunoglobulin (Ig)E (sIgE) are used to support a diagnosis of Hymenoptera venom hypersensitivity. The utility of venom allergen\u2010specific sIgE has not yet been evaluated for this purpose in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective of the study was to investigate the sensitivity (sn), specificity (sp) and positive predictive value (PPV) of honey bee and paper wasp serological titres using a commercially available sIgE assay [VARL (Veterinary Allergen Reference Laboratory) Liquid Gold] against clinical history for a diagnosis of Hymenoptera hypersensitivity in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Honeybee and paper wasp IgE serum titres were measured in 15 client\u2010owned dogs with a diagnosis of Hymenoptera venom hypersensitivity based on a history of anaphylaxis, owner observation of Hymenoptera, and positive IDT to bee and\/or wasp; and in 10 client\u2010owned dogs with no known history of anaphylaxis or Hymenoptera exposure and a negative IDT to bee and wasp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Analysis of receiver operating characteristic (ROC) curves demonstrate that a VARL score cut\u2010off of one of six for honeybee yields Sn, Sp and PPV of 40%, 60% and 60%, respectively, and two of six for wasp yields Sn, Sp and PPV of 25%, 78% and 60%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Owing to the poor sensitivity and PPV of sIgE assays for both bee and wasp hypersensitivity in dogs with known envenomation and anaphylaxis, the use of sIgE cannot be recommended as a tool for venom identification.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13194","type":"journal-article","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T02:14:27Z","timestamp":1690164867000},"page":"543-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sensitivity of a Hymenoptera serological immunoglobulin (Ig)E assay for the diagnosis of venom hypersensitivity in dogs"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1836-9135","authenticated-orcid":false,"given":"Hilary H.","family":"Chan","sequence":"first","affiliation":[{"name":"Animal Dermatology Clinic Perth The Animal Hospital Murdoch University  Murdoch Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda K.","family":"Burrows","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic Perth The Animal Hospital Murdoch University  Murdoch Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giselle","family":"Hosgood","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine The Animal Hospital Murdoch University  Murdoch Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudayna","family":"Ghubash","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic  Marina del Rey California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12342"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-4431.2009.00474.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/avj.13114"},{"key":"e_1_2_10_5_1","unstructured":"HoustonT.Native Bees.2011. 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