{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:37:53Z","timestamp":1790689073213,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T00:00:00Z","timestamp":1695600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Topical therapy is essential in assisting with the resolution of pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>(i) Evaluate the in vitro efficacy and residual activity of two different hair segments treated with shampoo and mousse against meticillin\u2010sensitive and meticillin\u2010resistant staphylococci; (ii) compare proximal and distal hair portions treated with the products and (iii) describe a new disc diffusion method for assessing residual efficacy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Eleven privately owned, medium\u2010haired dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In this randomised, blinded and negatively controlled study, dogs were treated once with a 3% chlorhexidine digluconate\u20100.5% ophytrium shampoo on the lateral thorax, and the corresponding mousse on the opposite side. Hairs were plucked before treatment, two\u2009hours post\u2010treatment, and day (D)2, D4, D7, D10 and D14. Hairs were weighed (0.01\u2009g) and cut (1.0\u2009cm) from the proximal portion, moistened with saline and placed on a sterile diffusion disc to absorb the solution. Proximal and distal hair bundles and diffusion discs were placed onto agar inoculated with an isolate of meticillin\u2010sensitive or meticillin\u2010resistant <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> or <jats:italic>Staphylococcus schleiferi<\/jats:italic>. Inhibition zones were measured following incubation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Distal hairs had larger (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) inhibition zones compared to proximal hairs. Mousse had significant differences (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) between time points and locations for both the hair bundles and discs, while shampoo only had a significant difference (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) between time points for the hairs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Mousse was effective, and shampoo was only minimally effective in inhibiting bacterial growth in vitro, with the greatest effect occurring at the two\u2009hours time point. The distal hair shafts had greater inhibition.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13206","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T03:31:12Z","timestamp":1695699072000},"page":"156-165","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In vitro efficacy of a topical antimicrobial mousse and shampoo at proximal and distal hair locations against <i>Staphylococcus pseudintermedius<\/i> and <i>Staphylococcus schleiferi<\/i>"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2397-0256","authenticated-orcid":false,"given":"Tia T.","family":"Taketa","sequence":"first","affiliation":[{"name":"Animal Dermatology Clinic  Tustin California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1616-8261","authenticated-orcid":false,"given":"Wayne S.","family":"Rosenkrantz","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic  Tustin California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig E.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic  Las Vegas Nevada USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willie A.","family":"Bidot","sequence":"additional","affiliation":[{"name":"Office of Animal Resources Western University of Health Sciences  Pomona California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,25]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"193","volume-title":"Muller and Kirk's small animal dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.2002.00298_13.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2006.00536.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.230.2.221"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12292"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12382"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01047.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01056.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12012"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12318"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12737"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12444"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12233"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.1994.tb00015.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01057.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2011.01002.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.579074"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-12-238"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","unstructured":"OllivierE ZemirlineC MarchandL ClossB.Effect of the ingredient A97614A1 on the adhesion and biofilm formation of staphylococcus pseudintermedius in amodelof reconstructed canine epidermis. 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