{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:45:54Z","timestamp":1778183154891,"version":"3.51.4"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T00:00:00Z","timestamp":1717545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cytological detection of acantholytic keratinocytes (acantholytic cells [AC]) helps to identify canine pemphigus foliaceus (cPF) yet AC also occurs in superficial pyoderma (SP), the main differential diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To compare selected cytomorphological features of cPF and SP and to establish cytological diagnostic criteria that could differentiate cPF from SP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>40 and 51 client\u2010owned dogs with PF and SP, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Impression smears from cPF (64), impetigo (40) and exfoliative superficial pyoderma (ESP) (17) samples were stained with Romanowsky stain, randomised, blinded and evaluated by two investigators independently. The entire sample was screened (\u00d7500 or \u00d71000 magnification) for round (AC1), boat (AC2) and raft AC, eosinophils and bacteria. Interobserver agreements were calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average number of the 10 highest \u00d7500 fields for AC1 and AC2 was significantly higher in PF than SP (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001; Kruskal\u2013Wallis test). Rafts and eosinophils were more common in PF than SP (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001; chi\u2010square test), while bacteria were rare in PF (5%; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001; chi\u2010square test). Observations between the experienced and novice investigators were highly correlated. An ROC analysis identified five AC1\/\u00d7500\u2010magnification field as a suitable cut\u2010off value for predicting PF diagnosis. This cut\u2010off value was tested by two additional investigators, who identified sensitivity of 84%\u2013100%, specificity of 95%\u201397% and accuracy of 95%\u201396% for the diagnosis of cPF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Criterion\u2010based impression smear cytological evaluation can provide strong evidence to support the clinical diagnosis. Acantholytic cell morphology varies in cPF and SP, and experience can improve accuracy in cytological differentiation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13267","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T05:54:16Z","timestamp":1717653256000},"page":"524-535","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of selected cytomorphological features of canine pemphigus foliaceus and superficial pyoderma"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2100-6418","authenticated-orcid":false,"given":"Tyler S.","family":"Spriggs","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie K. Y.","family":"Gedon","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith E.","family":"Linder","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8629-0528","authenticated-orcid":false,"given":"Petra","family":"Bizikova","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2006.00540.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13094"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098589403100103"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1002\/9780470752487","volume-title":"Skin diseases of the dog and cat","author":"Gross TL","year":"2005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12352"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1002\/9780470752487","volume-title":"Skin diseases of the dog and cat","author":"Gross TL","year":"2005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjem.2018.08.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000002864"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-41241-2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13174"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.104688"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-10722-2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00775.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/9780470752487","volume-title":"Skin diseases of the dog and cat","author":"Gross TL","year":"2005"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.13267","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T12:37:00Z","timestamp":1734611820000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.13267"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,5]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/vde.13267"],"URL":"https:\/\/doi.org\/10.1111\/vde.13267","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,5]]},"assertion":[{"value":"2024-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}