{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:43:25Z","timestamp":1772988205568,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Feline Bowenoid in\u00a0situ carcinoma (BISC) is frequently associated with\n                      <jats:italic>Felis catus<\/jats:italic>\n                      papillomavirus\u20102 (FcaPV\u20102). Although surgical excision of BISC is expected to be curative, recurrent lesions are reported and it is not known whether it is a consequence of incomplete surgery or residual viral load.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To combine colorimetric in\u00a0situ hybridisation (CISH) and quantitative (q)PCR for the detection of viral DNA, and to correlate the clinical outcome of cats with BISC in which FcaPV\u20102 DNA is detected at surgical margins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty\u2010seven cats with a histopathological diagnosis of BISC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Sections including core and margins of the lesions were used for histopathological evaluation, qPCR and CISH. After surgical removal of the lesion, clinical follow\u2010up data were recorded for 6\u2009months.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Six of 12 cases in which all four histological margins were evaluable were used to correlate the infection status at the margins with the follow\u2010up data. Four showed margin positivity, of which half relapsed as expected and half cured; two cases were negative, of which one cured as expected while the other relapsed. Fifteen cases where only three, two or one of the histological margins were evaluable, were considered to adequately correlate the status of infection with the follow\u2010up data if CISH was positive. Follow\u2010up data were available for three with positive margins: one relapsed while the other two were cured.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>Wide clinical surgical margins are always recommended for neoplastic conditions, yet there was no evidence that CISH margin examination would be beneficial in predicting recurrence in this viral\u2010induced lesion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.13297","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:38:45Z","timestamp":1725583125000},"page":"83-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Using colorimetric in\u00a0situ hybridisation method for\n                    <scp>FcaPV<\/scp>\n                    \u20102 to estimate postsurgical prognosis in feline Bowenoid in\u00a0situ carcinoma"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4414-4359","authenticated-orcid":false,"given":"Francesca","family":"Abramo","sequence":"first","affiliation":[{"name":"Department of Veterinary Science University of Pisa  Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3797-8320","authenticated-orcid":false,"given":"Maurizio","family":"Mazzei","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Pisa  Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Forzan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Pisa  Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giorgia","family":"Giannetti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Pisa  Pisa 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