{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T16:43:25Z","timestamp":1783183405507,"version":"3.54.6"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016986","name":"Nippon Zenyaku Kogyo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016986","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergen immunotherapy is used as aetiological treatment for canine atopic dermatitis (cAD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the anti\u2010inflammatory agent\u2010sparing effect over 1\u2009year of immunotherapy using pullulan\u2010conjugated recombinant Der f 2 (rDf2\u2010P).<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Twenty\u2010one privately owned dogs with cAD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Dogs with mild clinical signs after \u22654\u2009weeks of anti\u2010inflammatory drug treatment received rDf2\u2010P immunotherapy for 1\u2009year. A monthly medication score (MS) was calculated, and clinical signs were assessed using Canine Atopic Dermatitis Extent and Severity Index (CADESI)\u201004, cAD Eczema Area and Severity for Shiba Inu and pruritus scores. Serum thymus and activation\u2010regulated chemokine (TARC)\/C\u2010C Motif Chemokine Ligand\u201017 (CCL17) concentrations were assessed at the initial and final doses during the 6\u2010week induction phase and every 3\u2009months for 1\u2009year thereafter in 16 cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean MS decreased significantly by 43.0% (<jats:italic>p<\/jats:italic>\u2009=\u20090.022), 60.9% (<jats:italic>p<\/jats:italic>\u2009=\u20090.003), 70.0% (<jats:italic>p<\/jats:italic>\u2009=\u20090.0004), 58.7% (<jats:italic>p<\/jats:italic>\u2009=\u20090.0004) and 49.3% (<jats:italic>p<\/jats:italic>\u2009=\u20090.029) at 2.5, 4.5, 7.5, 10.5 and 13.5\u2009months, respectively. Drug\u2010sparing effects, assessed with MS and adjusted by clinical scores were rated as excellent for 12 (57.1%) and 11 (52.4%) dogs, good for 3 (14.3%) and 1 (4.8%) dogs, fair for 0 and 3 (14.3%) dogs, and poor for 6 (28.6%) and 5 (23.8%) dogs at 4.5 and 13.5\u2009months, respectively. The serum TARC\/CCL17 concentrations were significantly lower in the samples with lower CADESI\u201004 scores (&lt;17) than in those with higher CADESI\u201004 scores (&gt;17) (<jats:italic>p<\/jats:italic>\u2009=\u20090.0002).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Der f 2\u2010P immunotherapy can lead to a rapid reduction in anti\u2010inflammatory drug use and serve as an effective proactive therapy for cAD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13315","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T04:24:54Z","timestamp":1734409494000},"page":"443-452","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Immunotherapy using pullulan\u2010conjugated Der f 2 allergen\u00a0in canine atopic dermatitis: An anti\u2010inflammatory agent\u2010sparing 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