{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T11:54:32Z","timestamp":1780055672946,"version":"3.54.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Malassezia pachydermatis<\/jats:italic> (MP) is implicated in severe dermatitis and otitis externa (OE) of companion animals and recently gained attention for its increasing resistance to azole compounds. For this reason, developing novel therapeutic strategies is of great interest. In a previous work, we used reference yeast isolates to evaluate several compounds bearing acyl\/selenoureido moieties and primary\/secondary sulfonamide groups for antifungal activity through organic selenium and carbonic anhydrase inhibition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This work aimed to evaluate the antifungal efficacy of eight selenoureido compounds on 36 clinical MP isolates from dogs, compared to selected azoles, notably ketoconazole (KCZ), miconazole (MCZ) and fluconazole (FCZ).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>MIC assays of <jats:bold>5g<\/jats:bold>, <jats:bold>7a<\/jats:bold>, <jats:bold>7c<\/jats:bold>, <jats:bold>7k<\/jats:bold>, <jats:bold>8c<\/jats:bold>, <jats:bold>10c<\/jats:bold>, <jats:bold>11b<\/jats:bold>, <jats:bold>11f<\/jats:bold>, KCZ, MCZ and FCZ were performed on 36 MP field isolates isolated from dogs affected by dermatitis and\/or OE in which yeast aetiology was suspected. Minimum 50% and 90% inhibitory concentrations (MIC<jats:sub>50<\/jats:sub> and MIC<jats:sub>90<\/jats:sub>) were calculated. MP identification was confirmed with a nested PCR for the internal transcribed spacer region of the rRNA gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, the MIC<jats:sub>50<\/jats:sub> of the tested compounds on MP field isolates was higher than the MICs obtained on reference MP DSM 6172. Although KCZ showed the lowest MIC<jats:sub>50<\/jats:sub> value, compounds <jats:bold>5g<\/jats:bold>, <jats:bold>7a<\/jats:bold> and <jats:bold>7k<\/jats:bold> showed lower MIC<jats:sub>50<\/jats:sub>s than MCZ and FCZ. Five clinical isolates showed a MIC on azoles &gt;MIC<jats:sub>90<\/jats:sub>. Compounds <jats:bold>7a<\/jats:bold> (four of five), <jats:bold>10c<\/jats:bold> (three of five) and <jats:bold>8c<\/jats:bold> (three of five) showed lower MIC values on these isolates compared to the tested azoles, suggesting good activity in phenotypically azole\u2010resistant MP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Considering the increasing azole resistance of the <jats:italic>Malassezia<\/jats:italic> genus, selenoureido compounds could represent a potential topical treatment for dog skin and ear mycotic infections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13336","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T05:53:16Z","timestamp":1742190796000},"page":"302-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative efficacy of selenoureido carbonic anhydrase inhibitors and azole antifungal drugs against clinical isolates of <i>Malassezia pachydermatis<\/i>"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0211-5293","authenticated-orcid":false,"given":"Costanza","family":"Spadini","sequence":"first","affiliation":[{"name":"Department of Veterinary Science University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00f2","family":"Mezzasalma","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amienwanlen Eugene","family":"Odigie","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Bari \u201cAldo Moro\u201d  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Angeli","sequence":"additional","affiliation":[{"name":"Section of Pharmaceutical and Nutraceutical Science, NEUROFARBA Department University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Carta","sequence":"additional","affiliation":[{"name":"Section of Pharmaceutical and Nutraceutical Science, NEUROFARBA Department University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Selleri","sequence":"additional","affiliation":[{"name":"Section of Pharmaceutical and Nutraceutical Science, NEUROFARBA Department University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Gandolfo","sequence":"additional","affiliation":[{"name":"Veterinary Practitioner Veterinary Center \u201cGiuseppe Verdi\u201d  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Taddei","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Parma  Parma 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