{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T04:01:11Z","timestamp":1746763271904,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The submission of bacterial cultures to an off\u2010site microbiology laboratory is standard practice in veterinary medicine. The effects of a transport delay on culture results from canine pyoderma samples remain unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>The first objective was to evaluate the reliability of <jats:italic>Staphylococcus<\/jats:italic> species isolation by aerobic bacterial cultures from samples collected from canine pyoderma when transported to a microbiology laboratory on the collection day. The second objective was to evaluate the effect of a 72\u2010h transport delay on <jats:italic>Staphylococcus<\/jats:italic> spp. isolation and antibiotic susceptibilities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Thirty client\u2010owned dogs with pyoderma that presented to a private dermatology clinic were included in this study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Three sterile culturettes were used to sequentially swab one pyoderma lesion per dog. Two samples were transported on the collection day (immediate cultures), while one was refrigerated for 72\u2009h before transportation to a microbiology laboratory. Ninety cultures were performed and the results compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was good reliability of <jats:italic>Staphylococcus<\/jats:italic> spp. isolation between the immediate cultures. There was no significant difference in the <jats:italic>Staphylococcus<\/jats:italic> spp. isolation, <jats:italic>Staphylococcus<\/jats:italic> spp. methicillin resistance or <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> antibiotic susceptibilities with a 72\u2010h transport delay, with the exception of chloramphenicol susceptibilities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Cultures from canine pyoderma can have good reliability of <jats:italic>Staphylococcus<\/jats:italic> spp. isolation when submitted to a microbiology laboratory on the collection day. Cultures that are delayed at 4\u00b0C for \u226472\u2009h can still have reliable results regarding <jats:italic>S. pseudintermedius<\/jats:italic> isolation and susceptibilities.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13337","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T05:22:10Z","timestamp":1742448130000},"page":"343-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect on <i>Staphylococcus<\/i> species isolation from a 72\u2010h transport delay of canine pyoderma samples to a microbiology laboratory"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5948-4202","authenticated-orcid":false,"given":"L.","family":"Tongen","sequence":"first","affiliation":[{"name":"McKeever Dermatology Clinics  Eden Prairie Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Heinrich","sequence":"additional","affiliation":[{"name":"McKeever Dermatology Clinics  Eden Prairie Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mcgilliard","sequence":"additional","affiliation":[{"name":"School of Animal Sciences Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12979"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.102462"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11020348"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.04.002"},{"key":"e_1_2_11_6_1","first-page":"1084","article-title":"Prevalence of methicillin\u2010resistant staphylococci in canine pyoderma cases in primary care veterinary practices in Canada: a preliminary study","volume":"56","author":"Joffe D","year":"2015","journal-title":"Can Vet J"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12444"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780199855483.003.0004"},{"volume-title":"StatPearls","year":"2024","author":"Shreffler J","key":"e_1_2_11_9_1"},{"issue":"09","key":"e_1_2_11_10_1","first-page":"8540","article-title":"Pre\u2010analytical and post\u2010analytical errors in the clinical laboratory: a systematic review","volume":"13","author":"Dugad V","year":"2022","journal-title":"J Pharm Neg Results"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00110-14"},{"key":"e_1_2_11_12_1","unstructured":"TransPorter Product Insert & How to Use Swab Guide [Internet].2019Accessed Dec 8 2024. 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