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Differentially expressed genes in response to erythritol in JCM 2423 were identified by RNA\u2010Seq and quantitative reverse transcription\u2010PCR. The impact of trehalose, glucose and arginine supplementation on erythritol\u2010induced growth suppression of the 25 <jats:italic>S. hyicus<\/jats:italic> wild strains was also investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Erythritol suppressed the in\u00a0vitro growth of JCM 2423 and the 25 <jats:italic>S. hyicus<\/jats:italic> wild strains. Moreover, erythritol upregulated the transcription of multiple genes in JCM 2423, including those encoding ATP\u2010binding cassette transporter enzymes (<jats:italic>potB<\/jats:italic>, <jats:italic>potC<\/jats:italic> and <jats:italic>potD<\/jats:italic>), arginine biosynthetic pathway enzymes (<jats:italic>argF<\/jats:italic>, <jats:italic>argG<\/jats:italic> and <jats:italic>argH<\/jats:italic>), <jats:sc>l<\/jats:sc>\u2010arginine deiminase pathway enzymes <jats:italic>(arcA<\/jats:italic>, <jats:italic>arcC<\/jats:italic> and <jats:italic>arcD<\/jats:italic>), fatty acid metabolism pathway enzymes (<jats:italic>fabH<\/jats:italic> and <jats:italic>fabF<\/jats:italic>) and trehalose metabolism\u2010related proteins (<jats:italic>treC<\/jats:italic>, <jats:italic>treR<\/jats:italic> and <jats:italic>treP<\/jats:italic>). Supplementation of trehalose or glucose in aerobic conditions or arginine supplementation in anaerobic conditions restored in\u00a0vitro growth of the 25 <jats:italic>S. hyicus<\/jats:italic> wild strains treated by erythritol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Erythritol suppresses the in\u00a0vitro growth of <jats:italic>S. hyicus<\/jats:italic> by inhibiting intracellular sugar and arginine metabolism under aerobic and anaerobic conditions, respectively.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13364","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T07:19:01Z","timestamp":1750144741000},"page":"494-505","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Erythritol alters the expression of genes related to sugar and arginine metabolisms and suppresses the growth of <i>Staphylococcus hyicus<\/i>"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9303-283X","authenticated-orcid":false,"given":"Saki","family":"Onishi\u2010Sakamoto","sequence":"first","affiliation":[{"name":"Cooperative Division of Veterinary Sciences, Graduate School of Agriculture Tokyo University of Agriculture and Technology  Fuchu Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keito","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Cooperative Department of Veterinary Medicine, Faculty of Agriculture Tokyo University of Agriculture and Technology  Fuchu Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7466-895X","authenticated-orcid":false,"given":"Tadashi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Medical Research on Prebiotics and Probiotics Fujita Health University  Toyoake Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensyu","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Shokukanken Inc.  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