{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:09:43Z","timestamp":1778641783161,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003551","name":"Royal Canin","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003551","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Canine atopic dermatitis (cAD) is a multifactorial, inherited skin disease, estimated to affect \u2264\u200915% of dogs. Studies of skin messenger mRNA in cAD currently use invasive methods, including blood sampling and biopsy collection, whilst advances in human atopic dermatitis study methodology have demonstrated reliable use of minimally invasive skin sampling techniques for similar objectives.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To validate the use of the minimally invasive D\u2010Squame (tape stripping; TS) method for skin mRNA analysis in dogs, and to investigate the association of highly dysregulated mRNAs with clinical response in a canine model of cAD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Eight healthy beagles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Dogs were epicutaneously sensitised twice weekly for 7\u2009weeks (49\u2009days) using house dust mites (HDM;\n                      <jats:italic>Dermatophagoides farinae<\/jats:italic>\n                      ). The clinical response of individual dogs to sensitisation was categorised as low, average, or high. On Day 49, D\u2010Squame TS samples were obtained from sensitised and nonsensitised sites from each dog, and transcriptomic analyses were performed. RNA\u2010Seq data analyses were performed using R and\n                      <jats:sc>Bioconductor<\/jats:sc>\n                      with default parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparing sensitised and control sites, 210 of 12,545 expressed genes were most differentially expressed (fold\u2010change \u2265\u20092,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.05).\n                      <jats:italic>CD2<\/jats:italic>\n                      ,\n                      <jats:italic>CD3D\/E<\/jats:italic>\n                      ,\n                      <jats:italic>CD209<\/jats:italic>\n                      (T cell),\n                      <jats:italic>IL13<\/jats:italic>\n                      ,\n                      <jats:italic>TNFRSF4<\/jats:italic>\n                      ,\n                      <jats:italic>CCL17<\/jats:italic>\n                      (T helper 2 cell [Th2]),\n                      <jats:italic>FCER1A<\/jats:italic>\n                      and\n                      <jats:italic>CD80<\/jats:italic>\n                      (dendritic cells) were among the top 50 most dysregulated genes significantly correlating with sensitisation. Dysregulation of several key mRNAs, including those involved in the Th2 pathway, correlated with clinical response.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>This proof\u2010of\u2010concept study using a minimally invasive method to investigate skin mRNA in cAD lesions enables ethical research of immune biomarkers involved in cAD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.70042","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T08:34:26Z","timestamp":1765355666000},"page":"353-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessing D\u2010Squame as a Minimally Invasive Technique to Evaluate the Cutaneous Immune Response\n                    <scp>mRNA<\/scp>\n                    in a Dog Model of Canine Atopic Dermatitis"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7628-5886","authenticated-orcid":false,"given":"Xavier","family":"Langon","sequence":"first","affiliation":[{"name":"R&amp;D Dermatology Research Royal Canin  Aimargues France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7682-2452","authenticated-orcid":false,"given":"Marion","family":"Mosca","sequence":"additional","affiliation":[{"name":"Interactions Cells Environment, 2016. A104, VetAgro Sup Dermatology Department  Marcy L'\u00c9toile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nad\u00e8ge","family":"Milhau","sequence":"additional","affiliation":[{"name":"Interactions Cells Environment, 2016. A104, VetAgro Sup Dermatology Department  Marcy L'\u00c9toile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6769-0587","authenticated-orcid":false,"given":"M\u00e9lanie","family":"Legain","sequence":"additional","affiliation":[{"name":"Interactions Cells Environment, 2016. A104, VetAgro Sup Dermatology Department  Marcy L'\u00c9toile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"Id\u00e9e","sequence":"additional","affiliation":[{"name":"Interactions Cells Environment, 2016. A104, VetAgro Sup Dermatology Department  Marcy L'\u00c9toile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Pin","sequence":"additional","affiliation":[{"name":"Interactions Cells Environment, 2016. A104, VetAgro Sup Dermatology Department  Marcy L'\u00c9toile France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13183"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16183"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.10.018"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci4030037"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.07.012"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.10.037"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.03.037"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.08.049"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16086"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.01.001"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.05.048"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15845"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.10.046"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.01.042"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16121"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.03.003"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci11030109"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13159"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13061"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.552251"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-020-01169-3"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10090547"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12010004"},{"key":"e_1_2_11_25_1","first-page":"111","article-title":"Finding Alternatives: An Overview of the 3Rs and the Use of Animals in Research","volume":"87","author":"Robinson V.","year":"2005","journal-title":"School Science Review"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.05.117"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13307"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000764"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.13040"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.11.047"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59340-x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31286-1"},{"key":"e_1_2_11_34_1","article-title":"Characterization of the Pro\u2010Inflammatory and Pruritogenic Transcriptome in Experimental Acute Canine IgE\u2010Mediated Skin Lesions","volume":"30","author":"Blubaugh A.","year":"2019","journal-title":"Veterinary Dermatology"},{"key":"e_1_2_11_35_1","article-title":"Transcriptome Profiling of Spontaneous Canine Atopic Dermatitis Lesional and Nonlesional Skin Using Deep RNA Sequencing","volume":"32","author":"Blubaugh A.","year":"2021","journal-title":"Veterinary Dermatology"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01098.x"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.04.011"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.03.002"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11152298"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40660-0"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12944"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.70042","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vde.70042","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.70042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:19:39Z","timestamp":1778638779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.70042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,10]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/vde.70042"],"URL":"https:\/\/doi.org\/10.1111\/vde.70042","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,10]]},"assertion":[{"value":"2025-02-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}