{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:09:27Z","timestamp":1778641767677,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009231","name":"European Society of Veterinary Dermatology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009231","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Canine leishmaniosis (CanL) is a zoonotic disease of variable severity. The typical immune response is driven by an exaggerated humoral immune response. Protective immunity is mediated by pro\u2010inflammatory cytokines that enhance macrophage leishmanicidal activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the cutaneous and the systemic immune responses as well as the cutaneous parasitic load in affected dogs before and after 28\u2009days of treatment with meglumine antimoniate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twelve dogs with CanL and skin lesions, treated with meglumine antimoniate at a target dose of 100\u2009mg\/kg subcutaneously every 24\u2009h, were prospectively enrolled.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Materials<\/jats:title>\n                    <jats:p>Before and after 28\u2009days of treatments, blood samples and skin biopsies were collected. Circulating levels of host defence peptides, leptin and cytokines were determined via enzyme\u2010linked immunosorbent assay (ELISA). Paraffin\u2010embedded skin biopsies were processed for routine immunofluorescence and positive cells were identified using commercially available anti\u2010canine antibodies. Parasitic load also was determined via molecular methods. All variables were statistically analysed with the significance level set at 0.05.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among the cutaneous cell types investigated, there was a decrease in the number of T\u2010box transcription factor TBX21 (Tbet\n                      <jats:sup>+<\/jats:sup>\n                      ) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.016), GATA binding protein 3 (GATA3\n                      <jats:sup>+<\/jats:sup>\n                      ) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.016), interleukin (IL)\u201017A\n                      <jats:sup>+<\/jats:sup>\n                      (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.03) cells and neutrophils (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.008) after treatment, whereas there were no significant changes in forkhead box protein P3 (FoxP3\n                      <jats:sup>+<\/jats:sup>\n                      ) regulatory T and ionised calcium\u2010binding adaptor molecule 1 (Iba1\n                      <jats:sup>+<\/jats:sup>\n                      ) cells. A lack of change in serum concentration of inflammatory mediators was found. Finally, cutaneous parasitic load was significantly lower after treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.03).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>The results of this study show that the reduction of cutaneous parasitic load after meglumine antimoniate treatment results in downregulation of innate and adaptive cutaneous inflammatory responses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.70056","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T11:32:11Z","timestamp":1771414331000},"page":"455-463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Cutaneous Immunological Milieu Before and After Treatment With Meglumine Antimoniate in Dogs Naturally Affected by Leishmaniosis due to\n                    <i>Leishmania infantum<\/i>"],"prefix":"10.1111","volume":"37","author":[{"given":"M.","family":"Platenik","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Di Loria","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions University \u201cFederico II\u201d  Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Archer","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8308-3371","authenticated-orcid":false,"given":"M.","family":"Saridomichelakis","sequence":"additional","affiliation":[{"name":"Clinic of Medicine, Faculty of Veterinary Science University of Thessaly  Karditsa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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