{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:10:06Z","timestamp":1789171806534,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Canine cutaneous reactive histiocytosis (CRH) is a proliferative histiocytic disease characterised by the accumulation of dendritic cells (DCs). Differentiating CRH from other infectious or non\u2010infectious granulomatous or histiocyte\u2010rich diseases is challenging on histological evidence alone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>This study aims to characterise the predominant histiocytic population in 11 golden retrievers with histological features compatible with CRH, using ionised calcium\u2010binding adapter molecule 1 (Iba1) and a validated macrophage antibodies cocktail for canine skin diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Eleven golden retriever dogs were enrolled with a clinical and histological diagnosis of CRH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Paraffin\u2010embedded skin biopsy samples from cases with a confirmed diagnosis of CRH were evaluated to identify the histiocytic cell type. Two antibody reagents were used: (1) anti\u2010Iba1 (goat polyclonal), a broad histiocytic marker and (2) a cocktail of rabbit recombinant monoclonal antibodies against CD11b, CD68, CD163, CD14 and CD16 to phenotype cutaneous macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All cases showed positive immunostaining with both antibodies. Although Iba1 immunostaining was diffuse in most of the histiocytoid cell population, only a few scattered histiocytoid cells stained with the anti\u2010macrophage antibody cocktail.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Clinical Relevance<\/jats:title>\n                    <jats:p>Strong Iba1 positivity in the histiocytoid cells, together with their lack of staining for macrophage markers, supports a dendritic cell phenotype and is consistent with a diagnosis of CRH. In conclusion, using a macrophage marker in combination with a broad histiocytic marker may help to distinguish CRH from granulomatous, macrophage\u2010rich diseases in paraffin\u2010embedded skin tissue.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.70104","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T09:41:30Z","timestamp":1783330890000},"page":"745-751","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cutaneous Reactive Histiocytosis in Golden Retrievers: An\u00a0Immunohistochemical Approach"],"prefix":"10.1111","volume":"37","author":[{"given":"Francesco","family":"Albanese","sequence":"first","affiliation":[{"name":"Centro Dermatologico Veterinario Toscano  Arezzo 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