{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:05:22Z","timestamp":1790766322047,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Atopic dermatitis (AD) in dogs is a prevalent allergic disorder associated with environmental allergens, including the house dust mite\n                      <jats:italic>Dermatophagoides farinae<\/jats:italic>\n                      (Df).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>To describe clinical and immunological changes over time in dogs with canine (c)AD sensitised to Df undergoing Df allergen\u2010specific immunotherapy (ASIT) and to test the hypothesis that ASIT is associated with changes in Canine Atopic Dermatitis Extent and Severity Index, 4th iteration (CADESI\u201004), pruritus and medication scores and selected immunological markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Fifteen dogs with cAD sensitised to Df received ASIT for 12\u2009months. Clinical evaluations included CADESI\u201004 and pruritus Visual Analog Scale (PVAS). Gene expression of interleukin (IL)\u201031, interferon\u2010gamma (IFN\u2010\u03b3) and glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH) in peripheral blood mononuclear cells (PBMCs) was analysed using real\u2010time PCR. Serum levels of Df\u2010specific immunoglobulin (Ig)E, total IgG and IgG subclass 1 were measured using immunoassays. Evaluations were performed at 0, 6 and 12\u2009months.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Significant reductions in pruritus scores and IL\u201031 expression were observed, while IFN\u2010\u03b3 expression increased. CADESI\u201004 scores decreased, although the change was not statistically significant. A reduction in medication scores was observed over time. Serum analysis showed increased concentrations of IgG and IgG1, while Df\u2010specific IgE remained elevated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Dermatophagoides farinae<\/jats:italic>\n                      ASIT resulted in a reduction in pruritus and medication use over a 12\u2009month period. Associated with this, PBMCs IL\u201031 was reduced and IFN\u2010\u03b3 elevated. An increased serum IgG and IgG1 were measured, and Df\u2010specific IgE remained elevated. These findings support an immunological effect of ASIT, yet further studies with a larger cohort are required to validate the findings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.70110","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:31:02Z","timestamp":1785717062000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical and Immunological Responses to\n                    <i>Dermatophagoides farinae<\/i>\n                    Allergen Immunotherapy in Dogs With Atopic Dermatitis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2352-639X","authenticated-orcid":false,"given":"Jakaphan","family":"Wannawong","sequence":"first","affiliation":[{"name":"Prasu\u2010Arthorn Animal Hospital, Faculty of Veterinary Science Mahidol University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1259-6898","authenticated-orcid":false,"given":"Maturawan","family":"Tunhikorn","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Public Health, Faculty of Veterinary Science Mahidol University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9055-0940","authenticated-orcid":false,"given":"Pannathee","family":"Prangtaworn","sequence":"additional","affiliation":[{"name":"Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0482-0243","authenticated-orcid":false,"given":"Nawannaporn","family":"Saelim","sequence":"additional","affiliation":[{"name":"Siriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5150-3808","authenticated-orcid":false,"given":"Thapani","family":"Srisai","sequence":"additional","affiliation":[{"name":"Siriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-528X","authenticated-orcid":false,"given":"Nitaya","family":"Indrawattana","sequence":"additional","affiliation":[{"name":"Siriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"},{"name":"Biodesign Innovation Center, Department of Parasitology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"},{"name":"Biomedical Research Incubator Division, Research Department, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3960-4157","authenticated-orcid":false,"given":"Sivapong","family":"Sungpradit","sequence":"additional","affiliation":[{"name":"Department of Pre\u2010Clinic and Applied Animal Science, Faculty of Veterinary Science Mahidol University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5743-2240","authenticated-orcid":false,"given":"Anchalee","family":"Tungtrongchitr","sequence":"additional","affiliation":[{"name":"Siriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"},{"name":"Biodesign Innovation Center, Department of Parasitology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"},{"name":"Siriraj Dust Mite Center for Services and Research, Department of Parasitology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0058-0477","authenticated-orcid":false,"given":"Witthawat","family":"Wiriyarat","sequence":"additional","affiliation":[{"name":"Department of Pre\u2010Clinic and Applied Animal Science, Faculty of Veterinary Science Mahidol University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13192"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00346-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0515-5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12205"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0514-6"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2018.08.001"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00309-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00348-8"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01098.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12280"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.05.117"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00682.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines10071037"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00758.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.160.25.861"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00663"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-02768-2"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12967"},{"key":"e_1_2_10_21_1","first-page":"83","volume-title":"Allergic Skin Diseases of Dogs and Cats","author":"Reedy L. M.","year":"1997"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12107"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13138"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13159"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-90992-z"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1999.tb00372.x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.12.1300"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2004.06.003"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12284"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.254.11.1291"},{"key":"e_1_2_10_32_1","first-page":"15","article-title":"Canine Atopic Dermatitis: Prevalence, Impact, and Management Strategies","volume":"15","author":"Drechsler Y.","year":"2024","journal-title":"Veterinary Medicine (Auckland, N.Z.)"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(84)90282-3"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.1998.39.2.109"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62865-7"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2004.tb00222.x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2012.05.018"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.01.004"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.70110","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vde.70110","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.70110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:35:26Z","timestamp":1790764526000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.70110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,2]]},"references-count":37,"alternative-id":["10.1111\/vde.70110"],"URL":"https:\/\/doi.org\/10.1111\/vde.70110","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,2]]},"assertion":[{"value":"2025-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"vde.70110"}}