{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T19:01:30Z","timestamp":1773687690689,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,7,22]],"date-time":"2016-07-22T00:00:00Z","timestamp":1469145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Vet Emergen Crit Care"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine if the concentrations of ammonia and inflammatory mediators in feline stored whole blood (SWB) increase with duration of storage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective ex vivo study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>University Teaching Hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Thirteen cats, recruited from the hospital feline donor pool, deemed healthy based on the predonation donor screening process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interventions<\/jats:title><jats:p>One unit (30 mL) of whole blood was collected from 13 unique blood donor cats, anticoagulated with citrate\u2010phosphate\u2010dextrose, and stored at 4\u00b0C. Concentrations of ammonia, interleukin (IL) 6, and IL\u201010 were measured in 5 units weekly for 4 weeks. Presence of chemokine ligand (CXCL) 8 was measured weekly in 8 other units in the same manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements and Main Results<\/jats:title><jats:p>The ammonia concentration increased nonlinearly with duration of storage, from a median of 48 \u03bcmol\/L (range 25\u201374 \u03bcmol\/L) on day 0 and 417 \u03bcmol\/L (324\u2013457 \u03bcmol\/L) on day 28. IL\u20106 and IL\u201010 concentrations were below the lower limits of detection of the assay used (IL\u20106 &lt; 31.2 pg\/mL and IL\u201010 &lt; 125 pg\/mL). CXCL\u20108 was detected in 4 of 8 SWB units at all time points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Importance<\/jats:title><jats:p>Ammonia concentration increases with storage time in feline SWB. The clinical significance of this finding is yet to be determined. The presence of the proinflammatory chemokine CXCL\u20108 in feline SWB warrants further research to determine whether it can incite an inflammatory response in the recipient. Further research evaluating the epidemiology of transfusion reactions in cats should evaluate the effect of unit age, and should include the possible impact of the presence of CXCL\u20108.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vec.12510","type":"journal-article","created":{"date-parts":[[2016,7,22]],"date-time":"2016-07-22T19:12:43Z","timestamp":1469214763000},"page":"639-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The effect of storage on ammonia, cytokine, and chemokine concentrations in feline whole blood"],"prefix":"10.1111","volume":"26","author":[{"given":"Katherine A.","family":"Cummings","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Cummings School of Veterinary Medicine Tufts University  North Grafton MA 01536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda L.","family":"Abelson","sequence":"additional","affiliation":[{"name":"Department of 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