{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:08:15Z","timestamp":1747188495734,"version":"3.40.5"},"reference-count":8,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T00:00:00Z","timestamp":1627689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Vet Emergen Crit Care"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the use of a veterinary point\u2010of\u2010care urine culture system (POCUCS) for the diagnosis of septic peritonitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective feasibility study performed between August 2017 and April 2018.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Private referral hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Twenty samples of naturally occurring canine peritoneal effusion collected via aseptic abdominocentesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>Point\u2010of\u2010care urine culture systems were inoculated and incubated according to manufacturer's instructions. The presence of bacterial growth, estimation of colony\u2010forming units\/mL of bacteria, and organism identification were recorded. Bacterial growth and organism identification on POCUCS were compared to an aerobic culture performed at a commercial microbiology laboratory. Serial dilution and subsequent culture on a POCUCS of a confirmed<jats:italic>Escherichia coli<\/jats:italic>infected peritoneal effusion and negative control sample were performed to determine the lowest concentration of bacteria detectable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 10 septic and 10 aseptic samples of peritoneal effusion confirmed by aerobic laboratory culture. Of the 10 culture\u2010positive samples, 8 were culture\u2010positive on the POCUCS. The sensitivity and specificity of the POCUCS for the detection of bacteria in peritoneal effusion were 80.0% and 100%, respectively. The POCUCS lowest limit of detectable bacteria in peritoneal effusion was 1000 CFUs\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The POCUCS evaluated in this study was less sensitive and less rapid for diagnosing septic peritonitis than blood glucose to peritoneal effusion glucose ratio and plasma lactate to peritoneal effusion glucose ratio. This POCUCS is not recommended as a tool for diagnosing septic peritonitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vec.13098","type":"journal-article","created":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T19:26:03Z","timestamp":1627759563000},"page":"595-600","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Feasibility study evaluating a veterinary point\u2010of\u2010care urine culture system for diagnosing septic peritonitis"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9949-540X","authenticated-orcid":false,"given":"Brett R.","family":"Casna","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Carolina Veterinary Specialists Winston Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Simmerson","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Carolina Veterinary Specialists Winston Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sargurunathan","family":"Subashchandrabose","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology Wake Forest School of Medicine Winston Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,31]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1","volume-title":"Textbook of Veterinary Internal Medicine","author":"Ngwenyama TR","year":"2017"},{"key":"e_1_2_8_3_1","first-page":"E1","article-title":"Septic peritonitis: etiology, pathophysiology, and diagnosis","author":"Ragetly GR","year":"2011","journal-title":"Compend Contin Educ Vet"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.244.7.814"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.24.6.922-928.1986"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.13571"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"407","DOI":"10.2460\/javma.1994.204.03.407","article-title":"Postoperative complications and prognostic indicators in dogs and cats with septic peritonitis: 23 cases (1989\u20101992)","volume":"204","author":"King LG","year":"1994","journal-title":"Journal of the American Veterinary Medical Association"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/jvet.2003.50005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000002255"}],"container-title":["Journal of Veterinary Emergency and Critical Care"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vec.13098","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vec.13098","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vec.13098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T14:59:21Z","timestamp":1725548361000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vec.13098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,31]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/vec.13098"],"URL":"https:\/\/doi.org\/10.1111\/vec.13098","archive":["Portico"],"relation":{},"ISSN":["1479-3261","1476-4431"],"issn-type":[{"type":"print","value":"1479-3261"},{"type":"electronic","value":"1476-4431"}],"subject":[],"published":{"date-parts":[[2021,7,31]]},"assertion":[{"value":"2019-05-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}