{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:06:16Z","timestamp":1747188376339,"version":"3.40.5"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T00:00:00Z","timestamp":1652400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Vet Emergen Crit Care"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Captive reptiles often present with clinical signs suggestive of a clotting disorder or severe illness that can induce or exacerbate a coagulopathy. However, coagulopathies in reptiles are difficult to characterize due to lack of species\u2010appropriate reagents to perform coagulation tests. The objective of this study was to develop screening tests to evaluate the extrinsic and common pathways of coagulation in green iguanas (<jats:italic>Iguana iguana<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Findings<\/jats:title><jats:p>Reptile and avian thromboplastin, extracted from reptile and avian brains, respectively, were used to initiate coagulation in prothrombin time (PT) assays and commercially available reagents were used to determine Russell's viper venom time, thrombin time, and fibrinogen using the Clauss method. Coagulation assays were performed on citrate\u2010anticoagulated plasma from 18 healthy green iguanas. Results were summarized as median (minimum\u2013maximum): PT (reptile thromboplastin), 34.8 seconds (27.1\u201342.1 s), PT (avian thromboplastin), 78.5 seconds (51.6\u2013114.23 s), Russell's viper venom time, 56.15 seconds (18.4\u201379.7 s), thrombin time, 10 seconds (7.0\u201336.5 s), and fibrinogen, 258\u00a0mg\/dl (89\u2013563.0) (2.58 [0.89\u20135.63\u00a0g\/L]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>Commercial reagents can be used to evaluate the common pathway and fibrinogen; however, avian\u2010 or reptile\u2010sourced thromboplastin is preferred for a reliable coagulation trigger to perform the PT assay and evaluate the extrinsic pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vec.13201","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T15:58:45Z","timestamp":1652457525000},"page":"685-689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Coagulation testing in green iguanas (<i>Iguana iguana<\/i>) with development of prothrombin time assays using reptile and avian thromboplastin"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4339-1051","authenticated-orcid":false,"given":"Izidora","family":"Sladakovic","sequence":"first","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4299-2936","authenticated-orcid":false,"given":"Benjamin M.","family":"Brainard","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen J.","family":"Divers","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjory B.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Population Medicine and Diagnostic Sciences, College of Veterinary Medicine Cornell University  Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1638\/2008-0125.1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.5818\/1529-9651.13.3.20"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00024"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2009.04.011"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2004.05.004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.2009.74.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/vetreco-2017-000240"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0442.2002.00431.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2010.05.021"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(67)90008-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1309\/LM36LUSEDXEH0GCV"}],"container-title":["Journal 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