{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:56:20Z","timestamp":1773014180294,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T00:00:00Z","timestamp":1769385600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Vet Emergen Crit Care"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>In people, severe Hymenoptera stings can lead to systemic allergic reactions, acute kidney injury, rhabdomyolysis, hemolysis, shock, and even death. Hemolytic anemia as a result of multiple honeybee stings has been reported in dogs; however, there are no prior reports of hemolytic anemia due to wasp stings in the dog. In this report, we present a case of a dog that developed hemolytic anemia after a single wasp sting. We also report the vulnerability of RBCs to the wasp venom using an in vitro hemolysis assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case Summary<\/jats:title>\n                    <jats:p>\n                      A 3\u2010year\u2010old neutered male Yorkshire Terrier presented with hemoglobinuria 4\u00a0h after a suspected single wasp sting and achieved complete remission after a short course of treatment with glucocorticoids and IV infusion, without long\u2010term sequelae. An in vitro hemolysis assay using mastoparan, a major wasp venom constituent, was performed to test our hypothesis that increased sensitivity of the RBC membrane to wasp venom would lead to hemolysis after exposure to a small amount of venom by a single sting. Although no difference in the amount of mastoparan leading to hemolysis in 50% of the RBCs (HC\n                      <jats:sub>50<\/jats:sub>\n                      ) was seen between the patient and two healthy controls, the HC\n                      <jats:sub>50<\/jats:sub>\n                      was higher in whole blood samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>New and Unique Information<\/jats:title>\n                    <jats:p>This is the first report demonstrating that even a single wasp sting can cause hemolysis in dogs. In addition, based on our in vitro hemolysis assay, plasma may have an inhibitory effect on mastoparan\u2010induced hemolysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vec.70091","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T11:12:29Z","timestamp":1769425949000},"page":"132-137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mastoparan as a Potential Cause of Hemolytic Anemia Due to a Suspected Single Wasp Sting in a Dog"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4592-3090","authenticated-orcid":false,"given":"Shunya","family":"Yokota","sequence":"first","affiliation":[{"name":"Yuki Animal Hospital  Nagoya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroto","family":"Taira","sequence":"additional","affiliation":[{"name":"Yuki Animal Hospital  Nagoya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Ikezawa","sequence":"additional","affiliation":[{"name":"Animal Medical Technology  Nagoya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Ishii","sequence":"additional","affiliation":[{"name":"Animal Medical Technology  Nagoya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0803-0760","authenticated-orcid":false,"given":"Masashi","family":"Yuki","sequence":"additional","affiliation":[{"name":"Yuki Animal Hospital  Nagoya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins8020032"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13030206"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctsap.2006.10.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/JAA.S62288"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/0886022X.2023.2259230"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20105793(83)80385\u20108"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083164"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1999.214.07.1026"},{"issue":"1","key":"e_1_2_9_10_1","first-page":"29","article-title":"Bee Sting\u2010Induced Haemolysis, Spherocytosis and Neural Dysfunction in Three Dogs","volume":"61","author":"Wysoke J. 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