{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:32:39Z","timestamp":1787167959489,"version":"build-2736575974"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T00:00:00Z","timestamp":1389830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n \n The objective of the study is to evaluate the physical properties,\n in vitro<\/jats:italic>\n release profile, and antibacterial efficiency of chitosan films prepared with ofloxacin.\n <\/jats:p>\n <\/jats:sec>\n \n Procedure<\/jats:title>\n \n Mucoadhesive films were prepared by means of a casting and solvent evaporation technique performed in a 2\u00a0wt% acetic acid solution and distilled water. Physical properties were characterized by release and swelling studies, differential scanning calorimetry (\n DSC<\/jats:styled-content>\n ) analysis, and attenuated total reflectance Fourier transformed infrared spectroscopy (\n ATR<\/jats:styled-content>\n \u2010\n FTIR<\/jats:styled-content>\n ) analysis. The\n in vitro<\/jats:italic>\n evaluation of the films was performed by inhibiting\n Staphylococcus aureus<\/jats:italic>\n and\n Pseudomonas aeruginosa<\/jats:italic>\n through activity studies.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Circular ofloxacin\u2010loaded chitosan\u2010developed films with 0.3\u00a0mg of drug weighed 7\u00a0mg were 110\u00a0\u03bcm thick and 5\u00a0mm in diameter. The\n DSC<\/jats:styled-content>\n curve of ofloxacin\u2010loaded chitosan films suggests an amorphous dispersion of ofloxacin within these films.\n ATR<\/jats:styled-content>\n \u2010\n FTIR<\/jats:styled-content>\n analysis showed that ofloxacin is indeed present in the matrix film. The drug was released\n in vitro<\/jats:italic>\n over a 1\u2010h period. No statistical difference could be observed between the ofloxacin\u2010loaded chitosan films and sterile disk soaked for 1\u00a0min in 0.3% commercial ofloxacin ophthalmic solution for\n S.\u00a0aureus<\/jats:italic>\n and\n P.\u00a0aeruginosa<\/jats:italic>\n (\n P\u00a0<\/jats:italic>\n =\n \u00a0<\/jats:italic>\n 0.1686,\n P\u00a0<\/jats:italic>\n =\n \u00a0<\/jats:italic>\n 0.1172, respectively).The films presented a significantly larger mean bacterial inhibition zone of\n S.\u00a0aureus<\/jats:italic>\n than did the commercial ciprofloxacin control disk (\n P\u00a0=\u00a0<\/jats:italic>\n 0.0002) and a significantly larger mean bacterial kill zone of\n P.\u00a0aeruginosa<\/jats:italic>\n than did the commercial enrofloxacin control disk (\n P\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n 0.0001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Ofloxacin was successively incorporated onto chitosan films and was not inactivated during the process of manufacturing, thus preserving antibacterial proprieties.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vop.12140","type":"journal-article","created":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T02:42:52Z","timestamp":1389840172000},"page":"150-155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Mucoadhesive chitosan films as a potential ocular delivery system for ofloxacin: preliminary\n in vitro<\/i>\n studies"],"prefix":"10.1111","volume":"17","author":[{"given":"Gustavo","family":"de Oliveira Fulg\u00eancio","sequence":"first","affiliation":[{"name":"Ophthalmology Section School of Veterinary Medicine Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Ant\u00f4nio B.","family":"Viana","sequence":"additional","affiliation":[{"name":"Ophthalmology Section School of Veterinary Medicine Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Ot\u00e1vio S.","family":"Silva","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Carlos F.","family":"Lobato","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul R.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Agricultural Environmental and Biological Sciences Center of Federal University of Rec\u00f4ncavo of Bahia Cruz das Almas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju\u00e7ara R.","family":"Fanca","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Martins D.","family":"Byrro","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Augusto G.","family":"Faraco","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando","family":"da Silva Cunha\u2010J\u00fanior","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Federal University of Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2004.01.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.87.7.834"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(99)00003-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10731199009117286"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2011.0174"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2008.09.047"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/03639040701384942"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.228.1.80"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2008.0007"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00046-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-008-0228-1"}],"container-title":["Veterinary 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