{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:13:11Z","timestamp":1780416791065,"version":"3.54.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Impression cytology is a noninvasive investigation of the ocular surface. It uses the adhesive features of different filter papers to collect a monolayer of epithelial cells from the cornea and\/or conjunctiva. Samples obtained by impression cytology exhibit all characteristics of an ideal cytology specimen. The aim of this study was to test the feasibility of impression cytology and determine the most appropriate filter paper to achieve maximum diagnostic value of the feline eye.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals Studied<\/jats:title><jats:p>Ten healthy cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>The study was conducted in two phases. In the first phase, eight different filter papers (FPs) with various pore sizes were tested: 3.0\u2010, 1.2\u2010, 0.8\u2010, 0.45\u2010, 0.22\u2010, 0.05\u2010 and 0.025\u2010\u03bcm cellulose acetate papers and a 0.4\u2010\u03bcm Biopore membrane (<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>). Samples were obtained from the superior bulbar and from the inferior palpebral conjunctiva. In the second phase, three different sampling methods \u2013 with and without topical anesthesia, and with topical anesthesia and drying of the conjunctiva \u2013 were compared employing the<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>encased in the intended BM device (<jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content>). Samples were evaluated for cellularity and quality of cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the first phase, samples obtained from the superior bulbar conjunctiva with the<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>had the most sufficient cellularity and quality. In the second phase,<jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content>with topical anesthesia and additional drying of the conjunctiva was the most ideal method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The<jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content>may prove to be a suitable diagnostic tool for clinicians. Sampling is quick, processing is simple, and a large area of intact cells can be harvested.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12244","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T04:02:46Z","timestamp":1419912166000},"page":"276-284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Impression cytology: a novel sampling technique for conjunctival cytology of the feline eye"],"prefix":"10.1111","volume":"18","author":[{"given":"R\u00e9ka","family":"E\u00f6rd\u00f6gh","sequence":"first","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine Vienna Veterin\u00e4rplatz 1 Vienna A\u20101210 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Schwendenwein","sequence":"additional","affiliation":[{"name":"Department for Pathobiology University of Veterinary Medicine Vienna Veterin\u00e4rplatz 1 Vienna A\u20101210 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Tichy","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Veterinary Medicine Vienna Veterin\u00e4rplatz 1 Vienna A\u20101210 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Nell","sequence":"additional","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine Vienna Veterin\u00e4rplatz 1 Vienna A\u20101210 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1477","volume-title":"Veterinary Ophthalmology","author":"Stiles J","year":"2013"},{"key":"e_1_2_7_3_1","first-page":"69","volume-title":"Feline Ophthalmology","author":"Barnett KC","year":"1998"},{"key":"e_1_2_7_4_1","first-page":"83","article-title":"The benefit of conjunctival scraping cytology in the biological diagnosis of conjunctivitis in the dog and cat","volume":"3","author":"J\u00e9gou JP","year":"1993","journal-title":"European Journal of Companion Animal Practice"},{"key":"e_1_2_7_5_1","first-page":"533","volume-title":"Veterinary Ophthalmology","author":"Featherstone HJ","year":"2013"},{"key":"e_1_2_7_6_1","first-page":"74","article-title":"Conjunctival brush cytology: evaluation of a new cytological collection technique in dogs and cats with a comparison to conjunctival scraping","volume":"7","author":"Willis M","year":"1997","journal-title":"Veterinary Comparative Ophthalmology"},{"key":"e_1_2_7_7_1","first-page":"181","article-title":"Exfoliative cytology of conjunctiva and cornea in domestic animals: a comparison of four collecting techniques","volume":"6","author":"Bauer GA","year":"1996","journal-title":"Veterinary Comparative Ophthalmology"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(77)90499-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1983.01040020871007"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(85)33967-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.85.2.154"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1988.100"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-198801000-00012"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-6257(82)90115-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/112067210701700404"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1080\/15027732.1986.12461413","article-title":"Conjunctival morphology in Sj\u00f6gren's syndrome and other disorders of the anterior eye. 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