{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:00:14Z","timestamp":1788872414324,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,1,16]],"date-time":"2015-01-16T00:00:00Z","timestamp":1421366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Center for Equine Health, School of Veterinary Medicine, University of California-Davis","award":["V4S5302"],"award-info":[{"award-number":["V4S5302"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n To determine the role of equine herpesvirus (\n EHV<\/jats:styled-content>\n ) in idiopathic keratoconjunctivitis in horses and to determine whether sample collection method affects detection of\n EHV DNA<\/jats:styled-content>\n by quantitative polymerase chain reaction (q\n PCR<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Animals studied<\/jats:title>\n Twelve horses with idiopathic keratoconjunctivitis and six horses without signs of ophthalmic disease.<\/jats:p>\n <\/jats:sec>\n \n Procedures<\/jats:title>\n \n Conjunctival swabs, corneal scrapings, and conjunctival biopsies were collected from 18 horses: 12 clinical cases with idiopathic keratoconjunctivitis and six euthanized controls. In horses with both eyes involved, the samples were taken from the eye judged to be more severely affected. Samples were tested with q\n PCR<\/jats:styled-content>\n for\n EHV<\/jats:styled-content>\n \u20101,\n EHV<\/jats:styled-content>\n \u20102,\n EHV<\/jats:styled-content>\n \u20104, and\n EHV<\/jats:styled-content>\n \u20105\n DNA<\/jats:styled-content>\n . Quantity of\n EHV DNA<\/jats:styled-content>\n and viral replicative activity were compared between the two populations and among the different sampling techniques; relative sensitivities of the sampling techniques were determined.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Prevalence of\n EHV DNA<\/jats:styled-content>\n as assessed by q\n PCR<\/jats:styled-content>\n did not differ significantly between control horses and those with idiopathic keratoconjunctivitis. Sampling by conjunctival swab was more likely to yield viral\n DNA<\/jats:styled-content>\n as assessed by q\n PCR<\/jats:styled-content>\n than was conjunctival biopsy. EHV\u20101 and\n EHV<\/jats:styled-content>\n \u20104\n DNA<\/jats:styled-content>\n were not detected in either normal or\n IKC<\/jats:styled-content>\n \u2010affected horses;\n EHV<\/jats:styled-content>\n \u20102\n DNA<\/jats:styled-content>\n was detected in two of 12 affected horses but not in normal horses.\n EHV<\/jats:styled-content>\n \u20105\n DNA<\/jats:styled-content>\n was commonly found in ophthalmically normal horses and horses with idiopathic keratoconjunctivitis.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Because\n EHV<\/jats:styled-content>\n \u20105\n DNA<\/jats:styled-content>\n was commonly found in control horses and in horses with idiopathic keratoconjunctivitis, q\n PCR<\/jats:styled-content>\n was not useful for the etiological diagnosis of equine keratoconjunctivitis. Conjunctival swabs were significantly better at obtaining viral\n DNA<\/jats:styled-content>\n samples than conjunctival biopsy in horses in which\n EHV<\/jats:styled-content>\n \u20105\n DNA<\/jats:styled-content>\n was found.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vop.12250","type":"journal-article","created":{"date-parts":[[2015,1,16]],"date-time":"2015-01-16T03:29:49Z","timestamp":1421378989000},"page":"416-421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Detection of equine herpesvirus in horses with idiopathic keratoconjunctivitis and comparison of three sampling techniques"],"prefix":"10.1111","volume":"18","author":[{"given":"Steven R.","family":"Hollingsworth","sequence":"first","affiliation":[{"name":"Department of Surgical and Radiological Sciences University of California One Shields Avenue Davis CA 95616 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