{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:24:09Z","timestamp":1787016249726,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,3,28]],"date-time":"2015-03-28T00:00:00Z","timestamp":1427500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n \n To describe the tear ferning test (\n TFT<\/jats:styled-content>\n ) in healthy horses and its correlation with other parameters for evaluating the ocular surface.\n <\/jats:p>\n <\/jats:sec>\n \n Animals studied<\/jats:title>\n Thirty male and female adult healthy horses (60 eyes), of no defined breed.<\/jats:p>\n <\/jats:sec>\n \n Procedures<\/jats:title>\n \n Tear sample was collected with a microcapillary tube, placed on the surface of a glass slide, and allowed to dry at room temperature. The crystallization pattern was classified according to Rolando (\n Chibret International Journal Ophthamology<\/jats:italic>\n , 1984; 2, 32). The program\n STEP<\/jats:styled-content>\n anizer\n \u00a9<\/jats:sup>\n stereology tool, version 1.0, was utilized for counting points on the digitally captured crystallization image. A conjunctival biopsy was performed.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Tear ferning test was classified as Type I in 18 eyes (30%), Type\n II<\/jats:styled-content>\n in 31 eyes (51.7%), and Type\n III<\/jats:styled-content>\n in 11 eyes (18.3%), at a mean temperature of 27.3\u00a0\u00b1\u00a01.5\u00a0\u00b0C and relative humidity of 61.5\u00a0\u00b1\u00a05.7%. In the Type I crystallization, the count varied between 27 and 36 points (mean: 33.27\u00a0\u00b1\u00a02.40), in Type\n II<\/jats:styled-content>\n between 22 and 31 points (25.42\u00a0\u00b1\u00a01.95), and in Type\n III<\/jats:styled-content>\n between 13 and 25 points (16.82\u00a0\u00b1\u00a03.76). There was no statistical difference or correlation between the right and left eyes, nor was there a statistically significant influence (\n P<\/jats:italic>\n \u00a0<\u00a00.05) on\n TFT<\/jats:styled-content>\n by the factors evaluated. The mean goblet cells values were 50\u00a0\u00b1\u00a011.4 cells\/field. All samples showed the presence of lymphocytes, plasmocytes, and eosinophils.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Tear ferning test is easy to perform, without risks to the patient. Once standardized for horses, associated or not with the program\n STEP<\/jats:styled-content>\n anizer\n \u00a9<\/jats:sup>\n stereology tool, it is an additional method for evaluating the ocular surface.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vop.12268","type":"journal-article","created":{"date-parts":[[2015,3,29]],"date-time":"2015-03-29T02:34:16Z","timestamp":1427596456000},"page":"117-123","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Tear ferning test in horses and its correlation with ocular surface evaluation"],"prefix":"10.1111","volume":"19","author":[{"given":"Laura R.","family":"Silva","sequence":"first","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson F.","family":"Gouveia","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleyber J. T.","family":"de F\u00e1tima","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia B.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Janildo L.","family":"Reis","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato F.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Regimento de Pol\u00edcia Montada Coronel Rabelo of the Military Police of the Federal District Brasilia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepta M.","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula D.","family":"Galera","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Department College of Veterinary Medicine University of Bras\u00edlia Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,28]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"11","volume-title":"Ophthalmic Disease in Veterinary Medicine","author":"Martin CL","year":"2010"},{"key":"e_1_2_7_3_1","first-page":"1218","volume-title":"Veterinary Ophthalmology","author":"Brooks DE","year":"2007"},{"key":"e_1_2_7_4_1","first-page":"228","article-title":"Teste lacrimal de Schirmer em equinos da ra\u00e7a Mangalarga Marchador","volume":"3","author":"Chicharo F","year":"2010","journal-title":"Jornal Brasileiro de Ci\u00eancia Animal"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2011.184"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctep.2005.03.010"},{"key":"e_1_2_7_7_1","first-page":"438","volume-title":"Veterinary Ophthalmology","author":"Ollivier FJ","year":"2007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2004.07.002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-27492008000200017"},{"key":"e_1_2_7_10_1","first-page":"32","article-title":"Tear mucus ferning test in normal and keratoconjuntivitis sicca eyes","volume":"2","author":"Rolando M","year":"1984","journal-title":"Chibret International Journal Ophthamology"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1987.tb08501.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(82)34736-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000050838"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2010.03481.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2013000200021"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1475-1313.2008.00626.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0846-2.00001-X"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00137950"},{"key":"e_1_2_7_19_1","first-page":"203","volume-title":"The Preocular Tear Film in Health Disease, and Contact Lens Wear","author":"Rolando M","year":"1986"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199109000-00013"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/OPX.0b013e3180dc9a23"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1475-1313.2012.00915.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1475-1313.2000.00523.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e318259c934"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199401000-00010"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2008.0126"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2011.11.003"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clae.2013.09.011"},{"key":"e_1_2_7_29_1","first-page":"149","volume-title":"Diagnostic Cytology and Hematology of the dog and cat","author":"Yong KM","year":"2008"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvop.12268","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.12268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T18:52:43Z","timestamp":1696013563000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.12268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,28]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/vop.12268"],"URL":"https:\/\/doi.org\/10.1111\/vop.12268","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,28]]},"assertion":[{"value":"2015-03-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}