{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:28:59Z","timestamp":1790389739267,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T00:00:00Z","timestamp":1459987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the efficacy of topical 0.2% hyaluronic acid in canine corneal ulcers <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>Six purpose\u2010bred beagles were randomly assigned into two groups (three dogs\/group): group A received experimental product (Optimend<jats:sup>\u2122<\/jats:sup>, containing 0.2% hyaluronic acid, KineticVet<jats:sup>\u2122<\/jats:sup>); group B received control product (Optimend<jats:sup>\u2122<\/jats:sup> without 0.2% hyaluronic acid and supplemented with carboxymethylcellulose). The clinical scorer was masked to product content and subject assignment. Under sedation and topical anesthesia, 6\u2010mm axial corneal epithelial debridements were performed in the left eye. Wounded corneas received standard ulcer treatment and topical product (group A) or control product (group B) three times a day (<jats:styled-content style=\"fixed-case\">TID<\/jats:styled-content>) until ulcers were healed. Slit\u2010lamp biomicroscopy was performed 6 h after wounding and then every 12 h; findings were graded according to modified McDonald\u2013Shadduck scoring system; extraocular photography was performed after fluorescein stain application at all examination time points. Images were analyzed using <jats:styled-content style=\"fixed-case\">NIH <\/jats:styled-content><jats:sc>image j<\/jats:sc> software to quantify rate of corneal epithelialization. Gelatin zymography was used to analyze matrix metalloproteinase (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) 2 and 9 protein expression in tears collected at set time points during the study period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No statistical differences in clinical ophthalmic examination scores, rate of corneal epithelialization, or <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>2 or <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>9 protein expression were found between groups at any tested time point.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The application of 0.2% hyaluronic acid to standard ulcer medical management is well tolerated. Topical addition of the viscoelastic did not accelerate corneal wound healing compared to a topical control with similar viscosity in this study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12379","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T17:39:33Z","timestamp":1460309973000},"page":"123-130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Effects of topical hyaluronic acid on corneal wound healing in dogs: a pilot study"],"prefix":"10.1111","volume":"20","author":[{"given":"Kristina M.","family":"Gronkiewicz","sequence":"first","affiliation":[{"name":"Veterinary Medicine and Surgery University of Missouri Columbia, 900 East Campus Drive MO 65211 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Giuliano","sequence":"additional","affiliation":[{"name":"Veterinary Medicine and Surgery University of Missouri Columbia, 900 East Campus Drive MO 65211 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Sharma","sequence":"additional","affiliation":[{"name":"Veterinary Medicine and Surgery University of Missouri Columbia, 900 East Campus Drive MO 65211 USA"},{"name":"Harry S. Truman Memorial Veteran Hospital Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv R.","family":"Mohan","sequence":"additional","affiliation":[{"name":"Veterinary Medicine and Surgery University of Missouri Columbia, 900 East Campus Drive MO 65211 USA"},{"name":"Harry S. Truman Memorial Veteran Hospital Columbia MO USA"},{"name":"Mason Eye Institute School of Medicine Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463-5224.1998.00045.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-4431.2011.00654.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2003.027573"},{"key":"e_1_2_7_5_1","first-page":"18","article-title":"Hyaluronan, CD44 and fibronectin in rabbit corneal epithelial wound healing","volume":"40","author":"Asari A","year":"1996","journal-title":"Japanese Journal of Ophthalmology"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.12.019"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5853-x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000342200"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.55.503"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1993.1086"},{"key":"e_1_2_7_11_1","unstructured":"KallbergME BrooksDE OllivierFJet al.Differences in location of matrix metalloproteinases 2 and 9 in normal and ulcerated canine corneas (Abstract). 35th Annual Meeting of the American College of Veterinary Ophthalmologists;2004;Washington DC."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1990.83"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2010.00771.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3341\/kjo.1996.10.2.68"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/cxo.12073"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201200342"},{"key":"e_1_2_7_17_1","first-page":"2136","article-title":"The effect of chronic corneal epithelial debridement on epithelial and stromal morphology in dogs","volume":"43","author":"Bentley E","year":"2002","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_18_1","first-page":"1056","article-title":"A method to extract cytokines and matrix metalloproteinases from Schirmer strips and analyze using Luminex","volume":"17","author":"VanDerMeid KR","year":"2011","journal-title":"Molecular Vision"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2307\/2683975"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1207\/S15327906MBR3702_02"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00166757"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.08.023"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00137947"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.121533"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.81.1.80"},{"key":"e_1_2_7_26_1","first-page":"710","article-title":"Expression and distribution of adhesion molecule CD44 in healing corneal epithelia","volume":"39","author":"Yu FX","year":"1998","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-13730"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200405000-00015"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2005.21.376"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70613-4"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70128-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.014"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14-15512"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00396-008-1882-2"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12249-009-9266-6"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/OPX.0b013e3181824de3"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12886-015-0005-3"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-27492013000500008"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181e9adcc"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2007.00546.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.69.10.1341"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.16.4.289.10686"},{"key":"e_1_2_7_43_1","first-page":"1371","article-title":"In vivo bacterial protease production during Pseudomonas aeruginosa corneal infection","volume":"36","author":"Kernacki KA","year":"1995","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2001.0470"},{"key":"e_1_2_7_45_1","first-page":"1175","article-title":"Hyaluronic acid increases MMP\u20102 and MMP\u20109 expressions in cultured trabecular meshwork cells from patients with primary open\u2010angle glaucoma","volume":"18","author":"Guo MS","year":"2012","journal-title":"Molecular Vision"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/cbir.2001.0759"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-5155(00)00208-2"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.237.4.378"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2004.00327.x"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-014-2812-2"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0848"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvop.12379","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvop.12379","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.12379","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:09:26Z","timestamp":1695503366000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.12379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,7]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/vop.12379"],"URL":"https:\/\/doi.org\/10.1111\/vop.12379","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,7]]},"assertion":[{"value":"2016-04-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}