{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:39:08Z","timestamp":1758123548126},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,8,22]],"date-time":"2016-08-22T00:00:00Z","timestamp":1471824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the morphological details of the acini of the normal meibomian gland.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals Studied<\/jats:title><jats:p>Six young adult pigmented rabbits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The upper eyelid was prepared in extended configuration by glutaraldehyde fixation. Tissue block sections approximately 0.5\u20131\u00a0mm from the eyelid margin were assessed by light microscopy in sagittal sections and transmission electron microscopy (<jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>) in coronal sections. <jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content> images at between 1000\u00d7 and 2000\u00d7 magnification were enlarged onto A3\u2010sized prints and cell size and nuclei measured by planimetry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Light microscopy sagittal sections revealed clusters of variable sized acini, sometimes appearing to be slightly overlapping and without any obvious spatial organization of the internal cells (meibocytes). The estimated areas of the acini were close to 6500\u00a0sq micron. Coronal sections, as examined by <jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>, allowed for visualization of small to large acini (average diameter 82\u00a0\u00b1\u00a017 microns, with an estimated area of 5500\u00a0sq. microns) containing variable numbers of immature (partly differentiated) or mature (fully differentiated) meibocytes with a distinct spatial organization. The average area of the meibocytes was 158\u00a0\u00b1\u00a081 square microns, and they usually appeared to have a single nucleus (with an average sectional area of 29\u00a0\u00b1\u00a012 square microns). Within individual acini, peripherally located immature meibocytes tended to be smaller and have higher nucleo\u2010cytoplasmic area ratios, while more centrally mature located meibocytes tended to be slightly larger and had lower or much lower nucleocytoplasmic ratios.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Comparative studies on meibomian glands can be undertaken with objective assessments to assess for normality or abnormality.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12424","type":"journal-article","created":{"date-parts":[[2016,8,22]],"date-time":"2016-08-22T06:26:46Z","timestamp":1471847206000},"page":"335-343","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cell size and nucleo\u2010cytoplasmic ratios of meibocytes in the anterior acini of the upper eyelid Meibomian glands in rabbits"],"prefix":"10.1111","volume":"20","author":[{"given":"Michael 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