{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:54:58Z","timestamp":1771649698794,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T00:00:00Z","timestamp":1513555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007951","name":"Tokyo Medical and Dental University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007951","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate the anti\u2010inflammatory effect of rebamipide during corneal epithelial wound healing using a mouse wound repair model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A 2\u2010mm circular disc of the central cornea was demarcated in the right eye of C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice and the epithelium removed. Rebamipide 2% eyedrop was instilled onto the wounded eye 5 times a day (<jats:italic>n<\/jats:italic> = 26). Phosphate\u2010buffered saline (<jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>) was used in the control group (<jats:italic>n<\/jats:italic> = 26). Corneal and conjunctival <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 levels were measured at 6 h and 24 h postinjury by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. The wounded area was evaluated by fluorescein staining at 24 h postinjury. Macrophage infiltration was assessed immunohistochemically, and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 secretion from macrophages was examined <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Conjunctival <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 and corneal <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 levels were not significantly different between <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>\u2010treated and rebamipide\u2010treated groups. However, conjunctival <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 level was significantly lower in the rebamipide\u2010treated group compared with the <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>\u2010treated group. Macrophage migration into the conjunctiva, but not into the cornea, was suppressed by rebamipide treatment. In addition, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 secretion from cultured macrophages was suppressed by rebamipide in a concentration\u2010dependent manner. Rebamipide treatment significantly accelerated corneal epithelial wound healing at 24 h postinjury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In a mouse corneal epithelial wound model, rebamipide suppressed <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 secretion and macrophage infiltration in the conjunctiva, which might have contributed to accelerated corneal epithelial wound healing in the first 24 h following injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12510","type":"journal-article","created":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T13:27:55Z","timestamp":1513603675000},"page":"347-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Rebamipide suppresses <scp>TNF<\/scp>\u2010\u03b1 production and macrophage infiltration in the 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