{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T17:16:14Z","timestamp":1716398174176},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:00:00Z","timestamp":1524787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007709","name":"Michigan State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007709","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>Cairn terriers with ocular melanosis (OM<\/jats:styled-content>) accumulate large, heavily pigmented melanocytes in the anterior uvea. Darkly pigmented plaques develop within the sclera, leading us to hypothesize that OM<\/jats:styled-content> uveal melanocytes may have an abnormal migratory capacity.<\/jats:p><\/jats:sec>Animals studied<\/jats:title>Globes from OM<\/jats:styled-content>\u2010affected Cairn terriers and unaffected control eyes enucleated for reasons unrelated to this study were used for immunohistochemistry and to culture melanocytes for in\u00a0vitro cell behavior assays.<\/jats:p><\/jats:sec>Procedures<\/jats:title>The scleral plaques of six dogs were immunolabeled for HMB<\/jats:styled-content>\u201045, MelanA, PNL<\/jats:styled-content>2, CD<\/jats:styled-content>18, CD<\/jats:styled-content>204, and Iba\u20101 and compared with the pigment cells accumulated within the irides. Cultured uveal melanocytes from OM<\/jats:styled-content>\u2010affected and control dogs were compared using conventional assays measuring cell proliferation, invasion capability, and melanin production.<\/jats:p><\/jats:sec>Results<\/jats:title>Melanocytes isolated from OM<\/jats:styled-content> eyes had significantly elevated levels of per\u2010cell melanin content and production compared to controls. The majority of pigmented cells in the scleral plaques were HMB<\/jats:styled-content>45 positive indicating a melanocytic origin. Many were also CD<\/jats:styled-content>18 positive. No differences were observed between cultured melanocytes from OM<\/jats:styled-content>\u2010affected and control uvea for standard in\u00a0vitro proliferation or invasion assays.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Pigmented cells which accumulate in the sclera of OM<\/jats:styled-content>\u2010affected Cairn terriers are predominantly melanocytes; however, in\u00a0vitro assays of uveal melanocytes did not reveal differences in migratory behavior between OM<\/jats:styled-content> and control cells. Migratory behavior of OM<\/jats:styled-content>\u2010melanocytes may be environment\u2010dependent. We suggest that RNA<\/jats:styled-content> sequencing and differential expression analysis would be a useful next step in understanding this disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12572","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T12:12:23Z","timestamp":1524831143000},"page":"132-146","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Physiological characterization of ocular melanosis\u2010affected canine melanocytes"],"prefix":"10.1111","volume":"22","author":[{"given":"Ethan M.","family":"Dawson\u2010Baglien","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Genetics Program College of Natural Science Michigan State University Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica L.","family":"Noland","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health Michigan State University Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dodd G.","family":"Sledge","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health Michigan State University Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"Kiupel","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health Michigan State University Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon M.","family":"Petersen\u2010Jones","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Genetics Program College of Natural Science Michigan State University Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2007.00558.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2008.00640.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12208"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008390-199712000-00001"},{"key":"e_1_2_6_6_1","unstructured":"WeissteinEW.Least squares fitting.2002."},{"key":"e_1_2_6_7_1","first-page":"931","article-title":"Melanogenesis by human uveal melanocytes in\u00a0vitro","volume":"36","author":"Hu DN","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2007.00228.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.30"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113083"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90368-6"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000443877"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.037168"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0391"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/40.2.1552165"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-12-455"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvop.12572","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.12572","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T19:19:28Z","timestamp":1694719168000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.12572"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,27]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1111\/vop.12572"],"URL":"https:\/\/doi.org\/10.1111\/vop.12572","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,27]]},"assertion":[{"value":"2018-04-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}