{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:28:01Z","timestamp":1791286081903,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T00:00:00Z","timestamp":1524528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To compare corneal cytology samples from three common sampling techniques: cytobrush (<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>), Kimura platinum spatula (<jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>), and the handle edge of a scalpel blade (<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Equine patients presenting to the University of Florida College of Veterinary Medicine with ulcerative keratitis were included. Following diagnosis of corneal ulcer and sampling for microbial culture, two cytology samples per technique were collected with sterile<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>, and<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>in a randomized order. Cytologic evaluation was performed by two observers masked to collection method. Objective measures of sample cellularity, quality, distribution, and identification of infectious organisms were recorded per 10 monolayer cell populations using 50\u00d7 magnification with oil immersion which were compared to culture results. Variables were compared using<jats:styled-content style=\"fixed-case\">ANOVA<\/jats:styled-content>with Student's<jats:italic>t<\/jats:italic>test when appropriate and Cohen's kappa (k) to evaluate inter\u2010 and intra\u2010observer agreement (<jats:styled-content style=\"fixed-case\">IOA<\/jats:styled-content>) between observers and techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty equine patients (120 samples) were included. The<jats:styled-content style=\"fixed-case\">IOA<\/jats:styled-content>between observers was substantial (<jats:italic>k<\/jats:italic>= 0.75 \u00b1 0.06) for cytological parameters.<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>provided the most cellular samples (<jats:italic>P<\/jats:italic>&lt; .01). There was a trend toward agreement (<jats:italic>k<\/jats:italic>= 0.12 \u00b1 0.16) in technique for sample quality (<jats:italic>P<\/jats:italic>= .08).<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>had significantly poorer cell distribution than<jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>(<jats:italic>P<\/jats:italic>&lt; .05). Infection was confirmed in 12 of 20 patients with<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>techniques having a significantly higher diagnostic yield than<jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>(<jats:italic>P<\/jats:italic>&lt; .05) and was most consistent with infection confirmed on culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>provided the most diagnostic samples but all three techniques are clinically useful in evaluating equine ulcerative keratitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12574","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T09:27:49Z","timestamp":1524562069000},"page":"153-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Comparison of 3 corneal cytology collection methods for evaluating equine ulcerative keratitis: Cytobrush, kimura platinum spatula, and handle edge of scalpel blade"],"prefix":"10.1111","volume":"22","author":[{"given":"Laura","family":"Proietto","sequence":"first","affiliation":[{"name":"Departments of Small and Large Animal Clinical Sciences College of Veterinary Medicine University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah S.","family":"Beatty","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine College of Veterinary Medicine University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caryn E.","family":"Plummer","sequence":"additional","affiliation":[{"name":"Departments of Small and Large Animal Clinical Sciences College of Veterinary Medicine University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,24]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"81","volume-title":"Slatter's Fundamentals of Veterinary Ophthalmology","author":"Maggs DJ","year":"2007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12450"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0846-2.00001-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2010.00280.x"},{"key":"e_1_2_6_6_1","first-page":"43","volume-title":"Diagnostic Cytology and Hematology of the Horse","author":"Elizabeth A","year":"2002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1501\/Vetfak_0000002602","article-title":"Evaluation of brush cytology (cytospin technique) and cultural results in the diagnosis of keratoconjunctivitis in a goat herd","volume":"61","author":"Cakir LGK","year":"2014","journal-title":"Ankara Univ Vet Fak Derg"},{"key":"e_1_2_6_8_1","first-page":"533","volume-title":"Veterinary Ophthalmology","author":"Featherstone HJ","year":"2013"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0846-2.00002-1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2004.04.014"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199708)17:2<88::AID-DC2>3.0.CO;2-K"},{"key":"e_1_2_6_12_1","first-page":"1","article-title":"Examination of the feline eye and adnexa","volume":"17","author":"Crispin SM","year":"2007","journal-title":"EJCAP"},{"issue":"3","key":"e_1_2_6_13_1","first-page":"281","article-title":"Kate Robinson, Bruce H. 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