{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T23:13:57Z","timestamp":1714778037694},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T00:00:00Z","timestamp":1549238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate ocular and general safety of topical anti\u2010human VEGF bevacizumab and the effect on serum vascular endothelial growth factor (VEGF) values in healthy dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>Nine university\u2010owned beagles received 0.05\u00a0mL of 0.25% bevacizumab eyedrops (Avastin<jats:sup>\u00ae<\/jats:sup>, Roche) in one eye and 0.05\u00a0mL of 0.9% saline solution in the other eye as a control, administered at 12\u00a0hours intervals over a period of 28\u00a0days. Continuous monitoring for vital parameters and ocular examinations were conducted. Complete blood counts including hematology and coagulation parameters were performed before trial start as well as 24\u00a0hours, 7\u00a0days, and 28\u00a0days after trial start. Measurements of serum VEGF values were obtained using an ELISA\u2010based approach at days 0, 7, and 28. The experiment was designed as a masked placebo\u2010controlled study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No clinical signs of ocular toxicity or systemic incompatibility were noted in any dog at any time point of the study. No signs of pain were present in any dog at any time point. All blood count values remained in normal clinical ranges without relevant variation. There was no significant change in mean serum VEGF values between day 0 and day 7 and between day 0 and day 28.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results indicate that topical bevacizumab treatment is safe in healthy dogs. However, further studies are needed to assess safety and efficacy in diseased dogs with naturally occurring corneal neovascularization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12628","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T09:25:51Z","timestamp":1549358751000},"page":"600-606","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serum vascular endothelial growth factor changes and safety after topical anti\u2010human VEGF antibody bevacizumab in healthy dogs"],"prefix":"10.1111","volume":"22","author":[{"given":"Lisa\u2010Marie","family":"Muellerleile","sequence":"first","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Tichy","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Nell","sequence":"additional","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.243.5.667"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2006.00185.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1977.tb05852.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2011.01.041"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-198706040-00004"},{"issue":"12","key":"e_1_2_6_7_1","first-page":"2485","article-title":"Loss and restoration of immune privilege in eyes with corneal neovascularization","volume":"37","author":"Dana MR","year":"1996","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/178132"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2009.18"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2016.03.021"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.5935\/0004-2749.20140054"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2011.00973.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12102"},{"issue":"9","key":"e_1_2_6_14_1","first-page":"2514","article-title":"Expression of vascular endothelial growth factor and its receptors in finlamed and vascularized human corneas","volume":"41","author":"Philipp W","year":"2016","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1381"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2005.11.019"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2012.03.053"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2006.12.028"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2008.02.013"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-011-9249-6"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0420.2007.01049.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0570"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0420.2007.01067.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-007-0660-z"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2012-302451"},{"key":"e_1_2_6_26_1","volume-title":"Stabil\u2010Liste: Physikalisch\u2010chemische Stabilit\u00e4t, Kompatibilit\u00e4t und Inkompatbillit\u00e4t parenteral applizierbarer Zytostatika, Virustatika und Supportivtherapeutika","author":"Kr\u00e4mer I","year":"2013"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463-5224.2002.00243.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2011.00883.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2007.11.003"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2010.00846.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.71.5.570"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2012.01.007"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.12628","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vop.12628","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.12628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T12:46:02Z","timestamp":1694263562000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.12628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,4]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/vop.12628"],"URL":"https:\/\/doi.org\/10.1111\/vop.12628","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,4]]},"assertion":[{"value":"2018-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}