{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T19:28:44Z","timestamp":1780082924819,"version":"3.54.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T00:00:00Z","timestamp":1549238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009944","name":"ACVO Vision for Animals Foundation","doi-asserted-by":"publisher","award":["VAF2018-03"],"award-info":[{"award-number":["VAF2018-03"]}],"id":[{"id":"10.13039\/100009944","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007165","name":"University of Missouri","doi-asserted-by":"publisher","award":["DC860"],"award-info":[{"award-number":["DC860"]}],"id":[{"id":"10.13039\/100007165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007165","name":"University of Missouri","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007165","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To serially evaluate morphologic and elemental composition changes to diamond burr tips (DBTs) comparing two sterilization protocols.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals studied<\/jats:title><jats:p>A total of 300 fresh cadaver porcine globes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>Six DBTs were randomly, equally assigned into Group 1 or 2, and then analyzed using Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) at 0, 25, 50, and 100 cycles. Diamond burr debridement (DBD) was performed for 120\u00a0seconds on corneal stroma using the Algerbrush\u00ae. DBTs were cleaned, and then: Group 1 was sterilized by Germinator 500\u2122; and Group 2 underwent ultrasonic cleaning and pre\u2010vacuum autoclave. A cycle is defined as one DBD, cleaning and sterilization protocol. Data were quantified using custom MatLab program.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Energy Dispersive Spectroscopy revealed minor buildup of sulfur on both groups. Group 1 displayed major buildup of carbon and calcium. All DBTs were stippled with inorganic particulate at baseline. Particulates were no longer present on Group 2 by 25 cycles, but remained on Group 1 at all time points. There was significantly more buildup on Group 1 at all time points (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0000, 0.0009, and 0.0003 for 25, 50, and 100 cycles, respectively). More damage to Group 2 at all time points (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003, 0.002, and 0.003 for 25, 50, and 100 cycles, respectively) was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>No significant damage to Group 1 DBTs was noted after 100 cycles, however, particulate matter is not adequately removed using this sterilization technique. Ultrasonic cleaning is warranted between DBDs to achieve adequate particulate removal prior to sterilization; greater damage occurs with this technique which supports replacing DBTs regularly.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12632","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T09:24:32Z","timestamp":1549358672000},"page":"614-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of two cleaning and sterilization protocols of diamond burr tips used in debridement for canine superficial chronic corneal epithelial defects"],"prefix":"10.1111","volume":"22","author":[{"given":"Kayla C.","family":"Banks","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, College of Veterinary Medicine University of Missouri Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Stalla","sequence":"additional","affiliation":[{"name":"Electron Microscopy Core Facility University of Missouri Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filiz E.","family":"Bunyak","sequence":"additional","affiliation":[{"name":"Department of Computer Science University of Missouri Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommi A.","family":"White","sequence":"additional","affiliation":[{"name":"Electron Microscopy Core Facility University of Missouri Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loren G.","family":"Schultz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, College of Veterinary Medicine University of Missouri Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Giuliano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, College of Veterinary Medicine University of Missouri Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181a166b9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5326\/0410158"},{"issue":"10","key":"e_1_2_7_4_1","first-page":"2262","article-title":"Morphology and immunohistochemistry of spontaneous chronic corneal epithelial defects (SCCED) in dogs","volume":"42","author":"Bentley E","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-2867(03)90013-2"},{"issue":"7","key":"e_1_2_7_6_1","first-page":"2136","article-title":"The effect of chronic corneal epithelial debridement on epithelial and stromal morphology in dogs","volume":"43","author":"Bentley E","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ceo.12052"},{"issue":"3","key":"e_1_2_7_8_1","first-page":"627","article-title":"Persistent corneal ulcers. 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