{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T14:17:28Z","timestamp":1788617848291,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T00:00:00Z","timestamp":1549238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To describe the surgical technique and outcome of amniotic membrane transplantation (AMT) for treatment of complicated corneal ulcers in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals studied<\/jats:title><jats:p>One hundred and eleven dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Medical records (2010\u20102017) were reviewed to identify canine patients that underwent AMT for the treatment of complicated corneal ulcers. Quantitative and ordinal variables were analyzed using Mann\u2010Whitney <jats:italic>U<\/jats:italic> test and Fisher's Exact test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Brachycephalic breeds were overrepresented (74\/111; 66.6%). Three dogs were bilaterally affected. Fifty\u2010one had melting ulcers (51\/114; 44.7%), 33 stromal ulcers (33\/63; 52.4%), 17 descemetoceles (17\/63; 27%), and 13 perforations (13\/63;20.6%). Mean defect size was 6.2\u00a0mm (2\u201018\u00a0mm). Human (32\/114; 28.1%) or bovine (82\/114; 71.9%) (AmnioVet(R)) cryopreserved amniotic membranes were used. Monolayer (31\/114; 27.2%), bilayer (44\/114; 38.6%) or multilayer technique (39\/114; 34.2%) was performed, being anchored to the limbus (52\/114; 45.6%), to the defect (48\/114; 42.1%) or both (14\/114; 12.3%). Mean epithelial healing and follow\u2010up times were 25.6\u00a0days (15\u201045) and 98.7\u00a0days (21\u2010400), respectively. Most common postsurgical complications included pigmentation (10\/114; 8.8%) and graft failure (5\/114; 4.4%). Depth and size of corneal lesions, human amniotic membrane (AM) and concurrent ocular diseases were associated with more complications (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Good cosmetic and visual outcomes were achieved in 99.1% (113\/114) and 97.4% (111\/114) of cases, respectively. Zero to low graft opacity was seen in 23.9% of the eyes (27\/113), mild to moderate in 64.6% (73\/113) and complete in 11.5% (13\/113).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cryopreserved AMT is an effective surgical technique for the treatment of complicated corneal ulcers in the dog, with highly satisfactory visual and cosmetic outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12643","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T09:44:01Z","timestamp":1549359841000},"page":"695-702","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["A multicenter retrospective study on cryopreserved amniotic membrane transplantation for the treatment of complicated corneal ulcers in the dog"],"prefix":"10.1111","volume":"22","author":[{"given":"Daniel","family":"Costa","sequence":"first","affiliation":[{"name":"Servei d\u2019Oftalmologia de la Fundaci\u00f3 Hospital Cl\u00ednic Veterinari, Facultat de Veterin\u00e0ria Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Leiva","sequence":"additional","affiliation":[{"name":"Servei d\u2019Oftalmologia de la Fundaci\u00f3 Hospital Cl\u00ednic Veterinari, Facultat de Veterin\u00e0ria Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"},{"name":"Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Sanz","sequence":"additional","affiliation":[{"name":"Bellavista Visionvet Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Espejo","sequence":"additional","affiliation":[{"name":"Visualvet Bizkaia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Esteban","sequence":"additional","affiliation":[{"name":"Oftalmolog\u00eda Veterinaria Oca\u00f1a Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Vergara","sequence":"additional","affiliation":[{"name":"Hospital Veterinario Albeitar Logro\u00f1o Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinio","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Ocular Veterinaria Gran Canaria Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Huguet","sequence":"additional","affiliation":[{"name":"Oftalmovet Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00ed","family":"Cair\u00f3","sequence":"additional","affiliation":[{"name":"Servei d\u2019Oftalmologia de la Fundaci\u00f3 Hospital Cl\u00ednic Veterinari, Facultat de Veterin\u00e0ria Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"R\u00edos","sequence":"additional","affiliation":[{"name":"Laboratory of Biostatistics &amp; Epidemiology, Facultat de Medicina Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"},{"name":"Biostatistics and Medical Statistics Core Facility IDIBAPS, Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Servei d\u2019Oftalmologia de la Fundaci\u00f3 Hospital Cl\u00ednic Veterinari, Facultat de Veterin\u00e0ria Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"},{"name":"Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"872","article-title":"Microanatomy of the human amniotic membranes. 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