{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T12:34:41Z","timestamp":1769085281788,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T00:00:00Z","timestamp":1559088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Canine primary closed\u2010angle glaucoma (PCAG) is a complex disease caused by multiple genetic factors. A c.590G&gt;A variant in<jats:italic>OLFML3<\/jats:italic>was recently reported to be a candidate for pectinate ligament abnormality (PLA) and PCAG in the Border Collie. We investigated the association of this variant with PLA and PCAG in Border Collies from the United Kingdom.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The<jats:italic>OLFML3<\/jats:italic>variant was genotyped in 106 Border Collies comprising 90 with normal eyes (controls) and 16 with PLA (n\u00a0=\u00a011) and\/or PCAG (n\u00a0=\u00a05) (cases). Genotyping was performed in an additional 103 Border Collies to estimate variant frequency within the population. To investigate the association of the variant with disease in other breeds, genotyping was performed in 337 non\u2010Border Collies with PLA and\/or PCAG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 90 controls, 71 were homozygous for the wild\u2010type allele, two were homozygous for the variant, and 17 were heterozygous. Of the 16 cases, three were homozygous for the wild\u2010type allele, 11 were homozygous for the variant, and two were heterozygous. The association of the variant allele with disease was significant (<jats:italic>P<\/jats:italic>\u00a0=\u00a01.1\u00a0x\u00a010<jats:sup>\u22129<\/jats:sup>). We estimated the frequency of this variant to be 4.4% within the United Kingdom Border Collie population, and it was not identified in clinically affected dogs of any other breed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study confirms the association of the<jats:italic>OLFML3<\/jats:italic>variant with PLA and PCAG in Border Collies from the United Kingdom. DNA testing for the variant and selective breeding can reasonably be expected to result in a reduction of PLA and PCAG prevalence in the breed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12680","type":"journal-article","created":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T13:38:06Z","timestamp":1559137086000},"page":"25-36","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A variant in<i>OLFML3<\/i>is associated with pectinate ligament abnormality and primary closed\u2010angle glaucoma in Border Collies from the United Kingdom"],"prefix":"10.1111","volume":"23","author":[{"given":"James A. 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