{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:09:30Z","timestamp":1759936170994},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T00:00:00Z","timestamp":1569801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine whether four isolates of <jats:italic>Streptococcus canis<\/jats:italic> (<jats:italic>S\u00a0canis<\/jats:italic>) recovered from dogs diagnosed with ulcerative keratitis at the Animal Health Trust (AHT) were genetically related to other ocular isolates that are registered in the online database.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animal studied<\/jats:title><jats:p>Four <jats:italic>S\u00a0canis<\/jats:italic> corneal isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>Clinical and laboratory records between 2016 and 2017 were searched for dogs with ulcerative keratitis for which microbiology analysis was consistent with the growth of <jats:italic>S\u00a0canis<\/jats:italic>. Genomic DNA was extracted for sequencing (Illumina MiSeq), and multilocus sequence types (STs) were determined using MLST 1.8 relative to the 44 sequence types of <jats:italic>S\u00a0canis<\/jats:italic> available. A neighbor\u2010joining tree was constructed in MEGA v4.0. A two\u2010sided Fisher's exact test was used to determine any associations between the isolated strains and ocular infections of dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Four strains were isolated from pugs (cases 1\u20104) with ulcerative keratitis. Genome sequencing identified ST\u201027 (case 1), ST\u20109 (case 3), and ST\u201013 (cases 2 and 4). STs 13 and 27 are members of Clonal Complex (CC)\u201013. Analysis of the multilocus sequence typing database revealed that CC\u201013 strains accounted for six of the twelve isolates recovered from the eye exudates of dogs (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.0078).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>There is early evidence that the CC\u201013 group of <jats:italic>S\u00a0canis<\/jats:italic> is associated with ocular infections in dogs. We provide draft genome sequences toward the future identification of virulence mechanisms associated with streptococcal keratitis in dogs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12714","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T04:49:33Z","timestamp":1569905373000},"page":"252-258","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<i>Streptococcus canis<\/i> multilocus sequence typing in a case series of dogs with ulcerative keratitis"],"prefix":"10.1111","volume":"23","author":[{"given":"Andra E.","family":"Enache","sequence":"first","affiliation":[{"name":"Animal Health Trust Newmarket UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catriona","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Animal Health Trust Newmarket UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Kafarnik","sequence":"additional","affiliation":[{"name":"Animal Health Trust Newmarket UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew S.","family":"Waller","sequence":"additional","affiliation":[{"name":"Animal Health Trust Newmarket UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1996.tb00349.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985809359601"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2007.00996.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02912-12"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.12.013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idcr.2017.01.002"},{"key":"e_1_2_7_8_1","first-page":"41","article-title":"Human native endocarditis caused by Streptococcus canis\u2010a case report","volume":"127","author":"Mali\u0161ov\u00e1 B","year":"2018","journal-title":"Microbiol Immunol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2014.07.013"},{"issue":"2","key":"e_1_2_7_10_1","first-page":"59","article-title":"Streptococcus canis arthritis in a cat breeding colony","volume":"34","author":"Iglauer F","year":"1991","journal-title":"J Exp Anim Sci"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-9454"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(90)50053-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2005.04061.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2008.00579.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12599"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2007.00348.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12325"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463-5224.2000.3230121.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00246-09"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2016.11.011"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/23744235.2015.1043940"},{"key":"e_1_2_7_22_1","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals","author":"Clinical and Laboratory Standards Institute","year":"2018"},{"key":"e_1_2_7_23_1","unstructured":"The European Committee on Antimicrobial Susceptibility Testing.Breakpoint tables for interpretation of MICs and zone diameters. 2019. 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