{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T09:43:22Z","timestamp":1784627002920,"version":"3.55.0"},"reference-count":81,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007456","name":"Canine Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007456","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To introduce a protocol for the characterization of protein patterns in tears of dogs with primary angle closure glaucoma (PACG) and primary open\u2010angle glaucoma (POAG).<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Nineteen dogs (25 eyes).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tear samples were collected using a Schirmer tear strip, from dogs with PACG (PACG\u2010affected eyes, n\u00a0=\u00a08; unaffected eyes predisposed to PACG, n\u00a0=\u00a07), POAG (n\u00a0=\u00a04), and healthy controls (n\u00a0=\u00a06). Protein precipitation and trypsin digestion were performed for analyses via liquid chromatography\u2010tandem mass spectrometry. Proteins were identified using the SwissProt protein sequence database. Relative protein expression in 17 eyes (15 dogs) was evaluated using Proteome Discoverer 2.0. Pathway analyses were performed to investigate molecular mechanisms associated with primary glaucoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Unique peptides were identified in 505 proteins, with Major allergen Can f 1 and albumin identified with high confidence. Proteins unique to tears from diseased eyes (PACG: n\u00a0=\u00a07; POAG: n\u00a0=\u00a014) were identified. Nucleoside diphosphate was unique to tears in PACG eyes na\u00efve to therapy, while retinal binding protein and NSFL1 cofactor p47 were unique to medicated PACG eyes. Relative expression of 34 proteins differed between disease states. Pathway analyses identified that the \u2018inflammatory response\u2019 was among the top disease\/disorders in dogs with primary glaucoma (PACG and POAG) but not in healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Tear samples suitable for mass spectrometry were readily obtained from pet dogs without needing specialized equipment. Further studies to validate the findings and explore potential candidate biomarkers for early disease detection and potential therapeutic targets are indicated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12765","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T14:05:01Z","timestamp":1588601101000},"page":"131-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A technique for shotgun proteomic analysis of the precorneal tear film in dogs with naturally occurring primary glaucoma"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2548-2969","authenticated-orcid":false,"given":"Kathleen L.","family":"Graham","sequence":"first","affiliation":[{"name":"Clinical Ophthalmology and Eye Health Sydney Medical School University of Sydney Sydney NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eve","family":"Diefenbach","sequence":"additional","affiliation":[{"name":"Westmead Proteomics Facility Westmead Institute for Medical Research Westmead NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina I.","family":"McCowan","sequence":"additional","affiliation":[{"name":"University of Melbourne Veterinary Hospital University of Melbourne Melbourne Vic Australia"},{"name":"Department of Jobs, Precincts and Regions Victoria State Government Melbourne Vic Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J. 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