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In the wounded group, the axial corneal epithelium was removed by applying a 6\u00a0mm filter paper disk soaked in 1N\u2010NaOH for 60\u00a0s. Corneas were subsequently cultured using an air\u2010liquid interface model. Evaluation of corneal healing was performed daily, and culture medium was collected. Corneas were randomly assigned to undergo processing via histopathology and RNAscope in situ hybridization for interleukin\u20106 (IL\u20106) and alpha\u2010smooth muscle actin (\u03b1SMA) expression at T24, T48, and T72\u00a0h after wounding. Media of the cultured corneas were evaluated for the presence of lactate dehydrogenase (LDH) by a colorimetric assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The ulcerated area of the wounded corneas decreased over time and all corneas healed within 72\u00a0h. Histologically, normal corneal architecture was observed including healthy epithelium (in areas other than the ulcerated ones), minimal stromal edema, intact endothelium, and Descemet's membrane. IL\u20106 expression was increased in wounded corneas compared with unwounded controls. LDH expression was elevated for both wounded and unwounded corneas at T24 but decreased substantially and was not detected at T48 in media from wounded and unwounded corneas, respectively. No \u03b1SMA expression was detected from either wounded or unwounded corneas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The equine air\u2010liquid interface, <jats:italic>ex vivo<\/jats:italic>, corneal epithelial wound healing model is effective and physiologically relevant. This model can be used in future studies evaluating various corneal therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12935","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T13:40:58Z","timestamp":1632404458000},"page":"509-519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Interleukin\u20106 and lactate dehydrogenase expression in a novel <i>ex vivo<\/i> rocking model of equine corneal epithelial wound healing"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2417-5310","authenticated-orcid":false,"given":"Rita Fay","family":"Wehrman","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine Iowa State University  Ames Iowa 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