{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:52:40Z","timestamp":1787727160272,"version":"build-2784847793"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyze proteomic profiles of the aqueous humor (AH) of canines with primary angle\u2010closure glaucoma (PACG) and identify associated protein alterations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals studied<\/jats:title><jats:p>Six American Cocker Spaniels with PACG and six American Cocker Spaniels without ocular diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Aqueous humor samples were collected from six American Cocker Spaniels with PACG at Seoul National University, VMTH, and six healthy Cocker Spaniels without ocular disease at Irion Animal Hospital. For the PACG group, AH samples were obtained by anterior chamber paracentesis prior to glaucoma treatment. For the AH control group, AH samples were collected from patients anesthetized for other reasons. Total AH protein concentration was determined by the bicinchoninic acid (BCA) assay. AH protein samples were quantified by liquid chromatography\u2010mass spectrometry (LC\u2010MS\/MS). Raw MS spectra were processed using MaxQuant software 30, and the Gene Ontology (GO) enrichment analysis was performed using ClueGO.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The AH protein concentration in the PACG group (10.49\u00a0\u00b1\u00a017.98\u00a0\u00b5g\/\u00b5l) was significantly higher than that of the control group (0.45\u00a0\u00b1\u00a00.11\u00a0\u00b5g\/\u00b5l; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). A total of 758 proteins were identified in the AH. Several proteins both significantly increased (<jats:italic>n<\/jats:italic>\u00a0=\u00a069) and decreased (<jats:italic>n<\/jats:italic>\u00a0=\u00a0252) in the PACG group compared to those in the control group. GO enrichment analysis showed that the \u201cresponse to wounding,\u201d \u201cnegative regulation of endopeptidase activity,\u201d and \u201ccell growth\u201d pathways were the most enriched terms in the PACG group compared to the control group. The top 5 proteins that were significantly increased in the AH of the PACG group were secreted phosphoprotein 1 (SPP1), peptidoglycan recognition proteins 2 (PGLYRP2), tyrosine 3\u2010monooxygenase (YWHAE), maltase\u2010glucoamylase (MGAM), and vimentin (VIM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Gene Ontology enrichment analysis using the proteomic data showed that proteins and pathways related to inflammation were significantly upregulated in the various stage of PACG. Proteomic analysis of the AH from the PACG may provide valuable insights into PACG pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.12937","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T22:03:40Z","timestamp":1632521020000},"page":"520-532","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Proteomic analysis of aqueous humor in canine primary angle\u2010closure glaucoma in American Cocker Spaniel dogs"],"prefix":"10.1111","volume":"24","author":[{"given":"Seongjin","family":"Yun","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine and Research Institute for Veterinary Science Seoul National University Seoul Korea"},{"name":"Irion animal hospital Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dabin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of 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Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngsam","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine and Research Institute for Veterinary Science Seoul National University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je\u2010Yoel","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biochemistry BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6645-7116","authenticated-orcid":false,"given":"Kangmoon","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine and Research Institute for Veterinary Science Seoul 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