{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:40Z","timestamp":1747188460139,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate whether a commercially available amniotic membrane extract (AME) can accelerate corneal wound healing and suppress the early expression of MMP\u20109 in the tears of cats with experimentally induced superficial ulcerative keratitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedures<\/jats:title><jats:p>A total number of 16 cats were included. At the end of keratectomy, cats in the treatment group (TG, <jats:italic>n<\/jats:italic>\u00a0=\u00a08) received 40\u2009\u03bcl of AME (EyeQ\u00ae Amniotic Eye Drops, Vetrix\u00ae) four times daily, while cats in the control group (CG, <jats:italic>n<\/jats:italic>\u00a0=\u00a08) received 40\u2009\u03bcl of saline at the same time points. Tears were collected 24 and 48\u2009h after keratectomy, and the total MMP\u20109 was quantified by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The corneal re\u2010epithelialization rate did not differ between groups (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.26), being 0.48\u2009\u00b1\u20090.05\u2009mm<jats:sup>2<\/jats:sup>\/h in the CG and 0.41\u2009\u00b1\u20090.03\u2009mm<jats:sup>2<\/jats:sup>\/h in the TG. Similarly, the average time to achieve corneal wound healing did not differ between groups (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.25) and was 61.50\u2009\u00b1\u20093.54\u2009h in the CG and 70.50\u2009\u00b1\u20096.71\u2009h in the TG. The dimensions of the ulcerated areas also did not differ at any time point between the groups (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). In both groups, corneas healed without scarring, pigmentation, or vascularization. The expression of MMP\u20109 in the tears was similar in both groups at 24\u2009h post\u2010keratectomy, with a slight decrease at 48\u2009h (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The instillation of a commercial AME (EyeQ\u00ae) is safe, but it did not decrease the corneal re\u2010epithelialization time or the early expression of MMP\u20109 in the tears of cats with experimentally induced superficial ulcerative keratitis in this study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13024","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T13:16:15Z","timestamp":1662988575000},"page":"154-162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of commercial amniotic membrane extract on the re\u2010epithelialization time and the early expression of matrix metalloproteinase\u20109 in cats with experimentally induced corneal ulcers"],"prefix":"10.1111","volume":"26","author":[{"given":"Bruna Carvalho","family":"Silveira","sequence":"first","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria Universidade Federal de Mato Grosso  Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8922-2886","authenticated-orcid":false,"given":"Alexandre Pinto","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria Universidade Federal de Mato Grosso  Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio Dumit","family":"Pizzinatto","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria Universidade Federal de Mato Grosso  Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paloma Moraes","family":"Lobo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria Universidade Federal de Mato Grosso  Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna Rodrigues","family":"Miranda","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria Universidade Federal de Mato Grosso  Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalia","family":"de Assis Pereira","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria Universidade Federal de Mato Grosso  Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1477","volume-title":"Veterinary Ophthalmology","author":"Stiles 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