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The sensitivity of the NGS results was compared with bacterial and fungal culture results. Concordance between the NGS and culture results was determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The NGS results were positive for bacterial and fungal organisms in 5 and 1 normal and 18 and 1 IUK cases, respectively. Bacterial and fungal cultures were positive for 7 and 2 normal and 20 and 5 IUK cases, respectively. Sensitivity of NGS was 82.14% (95% confidence interval (CI), 63.11% to 93.94%) and specificity was 76.47% (95% CI, 50.10% to 93.19%). Concordance (complete and partial) between identified bacterial and fungal organisms was found in 79% and 100% of cases, respectively. NGS identified organisms in 3 culture\u2010negative IUK samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A commercial NGS service may be useful in the identification of causative agents in IUK cases with a sensitivity greater than the sensitivity previously reported for aerobic culture. Further testing is needed to determine the clinical significance of additional organisms isolated by NGS from infected cases, as well as organisms isolated from normal corneas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13069","type":"journal-article","created":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T16:30:51Z","timestamp":1679416251000},"page":"500-513","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of a commercial<scp>NGS<\/scp>service for detection of bacterial and fungal pathogens in infectious ulcerative keratitis"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0212-8134","authenticated-orcid":false,"given":"Ashley","family":"Bendlin","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne J.","family":"Gemensky\u2010Metzler","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dubraska","family":"Diaz\u2010Campos","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7521-7720","authenticated-orcid":false,"given":"Georgina M.","family":"Newbold","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather L.","family":"Chandler","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University Columbus Ohio USA"},{"name":"College of Optometry The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,21]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"976","volume-title":"Veterinary Ophthalmology","author":"Ledbetter EC","year":"2013"},{"key":"e_1_2_7_3_1","first-page":"252","volume-title":"Equine Ophthalmology","author":"Brooks DE","year":"2017"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0739(17)30136-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1998.tb04469.x"},{"key":"e_1_2_7_6_1","first-page":"600","article-title":"Bacterial and fungal isolates from equidae with ulcerative keratitis","volume":"182","author":"Moore CP","year":"1983","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(00)00179-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2003.00312.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12574"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.17.00434"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-21617"},{"key":"e_1_2_7_12_1","first-page":"533","volume-title":"Veterinary Ophthalmology","author":"Featherstone HJ","year":"2013"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2008.06.009"},{"key":"e_1_2_7_14_1","first-page":"68","article-title":"Laboratory diagnosis of keratomycosis: comparative evaluation of direct microscopy and culture results","volume":"25","author":"Vajpayee RB","year":"1993","journal-title":"Ann Ophthalmol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(96)30738-0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.2460\/javma.1999.215.11.1671","article-title":"Usefulness of aerobic microbial cultures and cytologic evaluation of corneal specimens in the diagnosis of infectious ulcerative keratitis in animals","volume":"215","author":"Massa K","year":"1999","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_7_17_1","unstructured":"JefferyU GervaisK MowatF HostetterS WhitleyD.Ability of corneal cytology to predict bacterial culture results. 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