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Samples were then assayed using the same substrate degradation assay, with recombinant activated matrix metalloproteinase\u20102 (MMP\u20102) added to measure protease inhibition effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For the protease activity assay, relative fluorescence (RF) for AH from normal pet dogs was 13.28\u2009\u00b1\u20092.25% of control collagenase while RF for AH from dogs with CPACG was 17.47\u2009\u00b1\u20094.67%; RF was 8.57\u2009\u00b1\u20091.72% for <jats:italic>ADAMTS10<\/jats:italic>\u2010OAG Beagles and 7.99\u2009\u00b1\u20091.15% for unaffected Beagles. For the MMP\u20102 inhibition assay, RF for AH from normal dogs was 34.96\u2009\u00b1\u200915.04% compared to MMP\u20102 controls, while RF from dogs with CPACG was 16.69\u2009\u00b1\u20097.95%; RF was 85.85\u2009\u00b1\u200913.23% for Beagles with <jats:italic>ADAMTS10<\/jats:italic>\u2010OAG and 94.51\u2009\u00b1\u20098.36% for unaffected Beagles. Significant differences were found between dogs with CPACG and both normal pet dogs and dogs with <jats:italic>ADAMTS10\u2010<\/jats:italic>OAG and between normal pet dogs and both groups of Beagles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>AH from dogs with CPACG is significantly more able to catalyze proteolysis and inhibit MMP\u20102 than AH from normal dogs or dogs with <jats:italic>ADAMTS10<\/jats:italic>\u2010OAG. Results suggest that pathogenesis may differ between CPACG and <jats:italic>ADAMTS10<\/jats:italic>\u2010OAG.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13143","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T03:36:05Z","timestamp":1693625765000},"page":"238-247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Relative ability of aqueous humor from dogs with and without primary angle\u2010closure glaucoma and <i>ADAMTS10<\/i> open\u2010angle glaucoma to catalyze or inhibit collagenolysis"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2084-7893","authenticated-orcid":false,"given":"Stephanie A.","family":"Pumphrey","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Cummings School of Veterinary Medicine at Tufts University  North Grafton Massachusetts 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