{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T16:07:02Z","timestamp":1792253222919,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T00:00:00Z","timestamp":1694995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The purpose of this case series was to describe the effect of autologous PRF membrane for corneal reconstruction surgery in dogs. PRF membranes made from two healthy dogs unrelated to the current case series were used for PRF histologic analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Seven dogs with complicated corneal ulcerations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Procedure<\/jats:title>\n                    <jats:p>\n                      A complete ophthalmic examination, hematology, and fibrinogen analysis were performed pre\u2010surgery. A PRF clot was made from autologous blood in a serum tube after centrifugation in a horizontal Bio\u2010PRF\u00ae Centrifuge at 700 \u00d7\n                      <jats:italic>g<\/jats:italic>\n                      for 8 min. The PRF clot was processed in a PRF\u2010Box\u00ae into a PRF membrane. The PRF membrane was sutured to the corneal ulcer bed. Each dog had a follow\u2010up at days 5\u20137, 12\u201314, and 30\u201340 post\u2010surgery. A final long\u2010term follow\u2010up was performed as well.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A positive outcome with healing and a \u201cgood\u201d quality PRF membrane was seen in six out of seven dogs. One dog had a fibrinogen level below normal range and the PRF membrane was of \u201cpoor\u201d quality. This dog developed a descemetocele 13\u2009days post\u2010surgery and needed rescue surgery. Mean healing time for all dogs was 9\u2009\u00b1\u20095.5\u2009days. Minimal scarring, corneal pigmentation, and vascularization were observed at the final long\u2010term follow\u2010up 288\u2009\u00b1\u200944\u2009days post\u2010surgery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PRF membrane was successful as graft material for corneal ulceration reconstruction surgery. Low fibrinogen appeared to have negative effect on the quality of the PRF membrane, showing the importance for the surgeon to evaluate the quality of the PRF membrane prior to surgery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vop.13148","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T13:58:15Z","timestamp":1695045495000},"page":"175-189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The use of a \u201chorizontal centrifugation protocol\u201d to prepare autologous platelet\u2010rich fibrin membranes for corneal reconstruction surgery in dogs with complicated corneal ulcerations: A case series"],"prefix":"10.1111","volume":"28","author":[{"given":"Louise","family":"Baadsgaard Bruun","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"},{"name":"Anicura Aarhus Veterinary Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommy A.","family":"Waskiw Hardon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"},{"name":"Haslev Veterinary Clinic  Haslev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne K.","family":"Havns\u00f8e Krogh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Qvortup","sequence":"additional","affiliation":[{"name":"Departmnent of Biomedical Science, Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen 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