{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:35:31Z","timestamp":1784288131958,"version":"3.55.0"},"reference-count":12,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate the efficacy of the Canine Cytokine SpikeMix\u2122 and MRM\u2010MS for detecting pro\u2010inflammatory cytokines in canine tears from healthy research Beagles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A complete ophthalmic examination was performed on 15 healthy research Beagles to verify no ophthalmic diseases. Tears were collected OU by placing a Weck\u2010Cel\u00ae cellulose spear in the ventral conjunctival fornix for 1\u2009min. The Weck\u2010Cel\u00ae spear was placed in a 2.0\u2009mL tube with a centrifuge filter forcing tears to flow through the filter into the bottom of the tube. The tears were analyzed using the Canine Cytokine SpikeMix\u2122 and MRM\u2010MS. Descriptive data from this study was reported as the normalized total peak area (nTPA) and median (range) using data imported from the online MRM\u2010MS Skyline program.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The level of 16 pro\u2010inflammatory cytokines was successfully detected in all 15 dogs. The four cytokines with the highest median amounts in the samples were IL\u20102\u2009=\u20090.1243 (0.019\u20136.7289), IL\u20106\u2009=\u20090.964 (0.0036\u201316.9365), TNF\u03b1\u2009=\u20090.1644 (0.0096\u20130.7138), and CSF\u20102\u2009=\u20090.4022 (0.1475\u20132.6208).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study revealed that 16 pro\u2010inflammatory cytokines in canine tears from healthy dogs can be detected with Canine Cytokine SpikeMix\u2122 and MRM\u2010MS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13156","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T14:09:38Z","timestamp":1698156578000},"page":"565-569","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Detection of pro\u2010inflammatory cytokines in healthy canine tears using Canine Cytokine <scp>SpikeMix<\/scp>\u2122 mass spectrometry via multiple reaction monitoring"],"prefix":"10.1111","volume":"26","author":[{"given":"Katya M.","family":"Spitznagel","sequence":"first","affiliation":[{"name":"Comparative Ophthalmology, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Railey","family":"Mikeska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haley","family":"Jost","sequence":"additional","affiliation":[{"name":"Comparative Ophthalmology, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"McGrath","sequence":"additional","affiliation":[{"name":"Neurology, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Mehaffy","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-7790","authenticated-orcid":false,"given":"Michala de Linde","family":"Henriksen","sequence":"additional","affiliation":[{"name":"Comparative Ophthalmology, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado USA"},{"name":"Comparative Ophthalmology, Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"issue":"10","key":"e_1_2_7_2_1","first-page":"2283","article-title":"Pro\u2010 and anti\u2010inflammatory forms of interleukin\u20101 in the tear fluid and conjunctiva of patients with dry\u2010eye disease","volume":"42","author":"Solomon A","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2013.195"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2020.110012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.4.4.248"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.09.008"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0273449"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-03080-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2008.00662.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","unstructured":"JostHE SpitznagelKM Corsato AlvarengaI et\u00a0al.Chronic administration of oral cannabidiol and its effect of tear production intraocular pressure and tear concentration in normal research Beagles. 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