{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T01:49:10Z","timestamp":1777600150964,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T00:00:00Z","timestamp":1714003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["D20FE\u2010305"],"award-info":[{"award-number":["D20FE\u2010305"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To survey the prevalence of pathogens in shelter\u2010housed cats with active ocular surface disease (OSD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals Studied<\/jats:title><jats:p>A total of 255 shelter\u2010housed domestic cats with evidence of active OSD. No normal, unaffected cats were sampled.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure(s)<\/jats:title><jats:p>OSD scoring was performed on cats with active OSD. Combined oropharyngeal\/conjunctival swabs were submitted for rt\u2010PCR\/PCR for feline herpesvirus (FHV\u20101), feline calicivirus (FCV), <jats:italic>Chlamydia<\/jats:italic> spp. (CHL), <jats:italic>Bordetella bronchiseptica<\/jats:italic> (BORD), and <jats:italic>Mycoplasma<\/jats:italic> spp. (MYC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pathogens were detected as follows: 76.4% (195\/255) MYC, 57.6% (147\/255) FHV\u20101, 42.7% (109\/255) FCV, 26.7% (68\/255) CHL, and 5.5% (14\/255) BORD. Monoinfections affected 21.1% (54\/255) animals, with MYC being the most common monoinfection (12.5%, 32\/255), followed by FHV\u20101 (4.7%, 12\/255), followed by CHL (2.4%, 6\/255), followed by FCV (1.6%, 4\/255), with no animals having a BORD monoinfection. Dual infections affected 36.4% of animals (93\/255), with MYC detected in 30.1% (77\/255) dual infections and FCV detected in 12.9% (33\/255) dual infections. Dual infections with MYC and FCV together were detected in 9.8% (25\/255) animals. Many animals (35.3%, 90\/255) were found to be affected by 3 or more pathogens, and 7.1% (18\/255) animals had no pathogens detected. OSD scores were not influenced by any variable assessed, including the number and type of pathogens detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MYC, FHV\u20101, FCV, and CHL were commonly detected in this group of animals with OSD. Both MYC and FCV (alone or in combination with each other) were detected in multiple animals with active OSD, supporting prior evidence that either may independently act as a primary ocular surface pathogen.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13218","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T14:40:46Z","timestamp":1714056046000},"page":"382-387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of <i>Mycoplasma<\/i> spp. and feline calicivirus in cats with ocular surface disease"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5671-3948","authenticated-orcid":false,"given":"Andrew C.","family":"Lewin","sequence":"first","affiliation":[{"name":"Veterinary Clinical Sciences, School of Veterinary Medicine Louisiana State University  Baton Rouge Louisiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2594-8968","authenticated-orcid":false,"given":"Xiaojuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Research Computing Support, Office of Innovative Technologies, University of Tennessee  Knoxville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13063"},{"key":"e_1_2_11_3_1","first-page":"1477","volume-title":"Veterinary Ophthalmology","author":"Stiles J","year":"2013"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006063"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.11.018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00185-5"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2010.03.006"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.6.643"},{"issue":"3","key":"e_1_2_11_9_1","first-page":"235","article-title":"Suceptibility of kittens to Mycoplasma felis infection","volume":"44","author":"Tan RJ","year":"1974","journal-title":"Jpn J Exp Med"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90045-H"},{"key":"e_1_2_11_11_1","first-page":"296","article-title":"Association of pleuropneumonia\u2010like organisms with conjunctivitis in cats","volume":"43","author":"Cello R","year":"1957","journal-title":"Am J Ophthalmol"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1973.tb06890.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(74)90065-6"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2011.00957.x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2012.00982.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00128-X"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13031"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.13218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T05:17:29Z","timestamp":1720588649000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.13218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,25]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/vop.13218"],"URL":"https:\/\/doi.org\/10.1111\/vop.13218","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,25]]},"assertion":[{"value":"2024-02-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}