{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T15:36:03Z","timestamp":1788622563153,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Identify microorganisms present in canine eyes affected by ulcerative keratitis and assess its resistance profile to available antimicrobial drugs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Samples were collected from 88 canine eyes that exhibited ulcerative keratitis. They were identified using MALDI\u2010TOF and subjected to antimicrobial susceptibility testing by disk diffusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among the assessed subjects, brachycephalic dogs accounted for 74.48% (50\/83) of the evaluated canines. Among the 88 evaluated eyes, 90.9% (80\/88) showed positive cultures, with 11.33% (10\/88) of the samples isolating more than one species of bacteria. Of all bacterial isolates identified (90), Gram\u2010positive bacteria accounted for 63.33% (57\/90), while Gram\u2010negative bacteria constituted 36.66% (33\/90), with predominance of\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      spp. at 35.55% (32\/90) being,\n                      <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                      at 68.75% (22\/32), and\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      at 15.55% (14\/90), respectively.\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      spp. exhibited resistance to penicillin (89.29%), sulfadiazine and trimethoprim (60.71%), and tetracycline (67.86%), while doxycycline (88.89%), cefotaxime (85.71%), chloramphenicol (82.14%), gentamicin, and moxifloxacin (78.57%) showed the highest sensitivity rates.\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      displayed sensitivity (100%) to gentamicin and imipenem, and resistance (8.33%) to norfloxacin, ciprofloxacin, and cefepime. Similarly, the Enterobacteriaceae family showed higher sensitivity to amikacin and gentamicin (88.89%), imipenem (88.24%), and levofloxacin (87.5%), with pronounced resistance to amoxicillin\u2010clavulanate (50%) and cefazolin (47.06%). This highlights multiresistance in 23.33% (21\/90) of the isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The most isolated species in canine ulcerative keratitis are\n                      <jats:italic>S. pseudintermedius<\/jats:italic>\n                      and\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      . However, other species were also isolated, demonstrating diversity in ocular microbiota infection. There is a high\u2010rate multidrug resistance associated with canine ulcerative keratitis. Nevertheless, these strains exhibited sensitivity to antimicrobials commonly used in veterinary ophthalmology.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vop.13224","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T14:05:52Z","timestamp":1716213952000},"page":"37-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence and antibiotic resistance in bacterial isolates of dogs with ulcerative keratitis in S\u00e3o Paulo State, Brazil"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5120-3041","authenticated-orcid":false,"given":"Pamella A. 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