{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T15:24:01Z","timestamp":1771860241070,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate the use of vital dyes and light microscopy for assessing canine corneal endothelial morphology ex\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The corneas of 40 canine eyes (<jats:italic>n<\/jats:italic>\u2009=\u200920 dogs) enucleated &lt;24\u2009h following euthanasia or death were isolated and flat\u2010mounted on a slide. Corneal endothelium was stained via 0.25% trypan blue followed by 0.5% alizarin red (pH\u20094.2), photographed, then the following morphological features were calculated using ImageJ: mean cell density (MCD), mean cell area (MCA), polymegathism (coefficient of variation of cell area), and pleomorphism (% hexagonality).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean\u2009\u00b1\u2009standard deviation (range) outcomes were: MCD, 2544\u2009\u00b1\u2009541 cells\/mm<jats:sup>2<\/jats:sup> (1750\u20133922 cells\/mm<jats:sup>2<\/jats:sup>); MCA, 431\u2009\u00b1\u200997\u2009\u03bcm<jats:sup>2<\/jats:sup> (251\u2013626\u2009\u03bcm<jats:sup>2<\/jats:sup>); polymegathism, 17\u2009\u00b1\u20092% (14%\u201322%); pleomorphism, 84\u2009\u00b1\u20093% (80%\u201390%). No significant differences (<jats:italic>p<\/jats:italic>\u2009\u2265\u2009.122) were noted for any outcome between male versus female or brachycephalic versus non\u2010brachycephalic dogs. Young dogs (&lt;10\u2009years) had lower MCA (<jats:italic>p<\/jats:italic>\u2009=\u2009.044), lower pleomorphism (<jats:italic>p<\/jats:italic>\u2009=\u2009.003), and higher MCD (<jats:italic>p<\/jats:italic>\u2009=\u2009.035) when compared to older dogs (\u226510\u2009years). Age was significantly (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.049) correlated with MCA (<jats:italic>r<\/jats:italic>\u2009=\u20090.467), MCD (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.476), polymegathism (<jats:italic>r<\/jats:italic>\u2009=\u20090.444), and pleomorphism (<jats:italic>r<\/jats:italic>\u2009=\u20090.609).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The combination of vital dyes and light microscopy allowed for clear visualization and evaluation of the corneal endothelium in canine eyes ex\u00a0vivo. Our findings can be used in future studies to deepen our understanding of the corneal endothelium in health and disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13260","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T04:54:16Z","timestamp":1721105656000},"page":"675-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Canine corneal endothelial cell analysis using vital dyes and light microscopy"],"prefix":"10.1111","volume":"28","author":[{"given":"Yamit","family":"Soueid","sequence":"first","affiliation":[{"name":"Koret School of Veterinary Medicine The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaden","family":"Baransy","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine The Hebrew University of Jerusalem  Rehovot 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