{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T07:23:11Z","timestamp":1787383391697,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["D20FE\u2010305"],"award-info":[{"award-number":["D20FE\u2010305"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the replication kinetics and cytopathic effect (CPE) of feline calicivirus (FCV) in feline corneal epithelial cells (FCEC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals Studied<\/jats:title>\n                    <jats:p>Seven archived FCV isolates and one archived feline herpesvirus type 1 (FHV\u20101) isolate, previously obtained from eight domestic short hair cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Procedures<\/jats:title>\n                    <jats:p>FCV RNA was extracted for sequencing using Illumina MiSeq, to identify three genomically diverse isolates for further testing. Following reference\u2010based assembly, viral genomes were annotated and assessed. Superficial keratectomies were performed to isolate the corneal epithelium of cats and the cells were cultured in\u00a0vitro. FCEC were infected with the three chosen FCV isolates and one FHV\u20101 isolate at two different multiplicity of infection ratios (MOIs, 0.1 and 0.01 PFU\/cell) and virus titration was assessed at 0, 2, 6, 12, 24, and 48\u2009h post\u2010infection (hpi). Viral identity was confirmed by RT\u2010qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Three genomically diverse FCV isolates were chosen for further assessment in the FCEC model. All infections of FCEC with FCV led to visible CPE, characterized by epithelial cell rounding and detachment from the plate by 24 hpi, while FHV\u20101 led to visible CPE within 48 hpi. All three of the FCV isolates replicated effectively in FCEC at both 0.1 and 0.01 MOI, with a peak increase in titer approximately 12\u201324 hpi.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results support the possible role of FCV as a primary pathogen of the feline ocular surface. FCV replicates in FCEC in\u00a0vitro, leading to profound CPE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vop.13289","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T21:48:37Z","timestamp":1728424117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Replication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells"],"prefix":"10.1111","volume":"29","author":[{"given":"Daniela I.","family":"Saade","sequence":"first","affiliation":[{"name":"Veterinary Clinical Sciences School of Veterinary Medicine, Louisiana State University  Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin\u2010Chi","family":"Liu","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Sciences School of Veterinary Medicine, Louisiana State University  Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erinn P.","family":"Mills","sequence":"additional","affiliation":[{"name":"William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent","family":"Stanfield","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences School of Veterinary Medicine, Louisiana State University  Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7402-5989","authenticated-orcid":false,"given":"C\u00f4me J.","family":"Thieulent","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences School of Veterinary Medicine, Louisiana State University  Baton Rouge Louisiana USA"},{"name":"Louisiana Animal Disease Diagnostic Laboratory School of Veterinary Medicine, Louisiana State University  Baton Rouge Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir 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