{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T07:23:11Z","timestamp":1787383391967,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n \n To investigate the level of pro\u2010inflammatory cytokines in aqueous humor from\n ADAMTS10<\/jats:italic>\n \u2010mutant research Beagles at an early stage of open\u2010angle glaucoma (OAG).\n <\/jats:p>\n <\/jats:sec>\n \n Animals Studied<\/jats:title>\n \n Twenty\u2010four research Beagles were enrolled into this case\u2010controlled study. Three groups of\n ADAMTS10<\/jats:italic>\n \u2010mutant research Beagles were enrolled in this study: (1) Intraocular pressure (IOP) 15\u201325\u2009mmHg (normotensive IOP), (2) IOP 25\u201330\u2009mmHg, and (3) IOP 30\u201336\u2009mmHg. An unaffected control group of Beagles that were either\n ADAMTS10<\/jats:italic>\n \u2010wildtype (WT) or heterozygote\u2010carrier with normotensive IOPs were also enrolled.\n <\/jats:p>\n <\/jats:sec>\n \n Procedures<\/jats:title>\n \n Aqueous humor samples were collected and analyzed for 16 pro\u2010inflammatory cytokines using the Canine Cytokine SpikeMix and target mass spectrometry via multiple reaction monitoring (MRM\u2010MS). Statistical differences between the four groups' pro\u2010inflammatory cytokines, as well as correlations between pro\u2010inflammatory cytokines and IOPs, were analyzed using Kruskal\u2010Wallis tests and Spearman's rho (\n \u03c1<\/jats:italic>\n ) correlations, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n IOP 15\u201325\u2009mmHg (\n n<\/jats:italic>\n \u2009=\u20098); IOP 25\u201330\u2009mmHg (\n n<\/jats:italic>\n \u2009=\u20096); IOP 30\u201336\u2009mmHg (\n n<\/jats:italic>\n \u2009=\u20095); and Control (WT\n n<\/jats:italic>\n \u2009=\u20093, carrier\n n<\/jats:italic>\n \u2009=\u20092, total\n n<\/jats:italic>\n \u2009=\u20095). There were no differences between pro\u2010inflammatory cytokines across the four groups (all\n p<\/jats:italic>\n \u2010values >\u20090.05). IL\u201013 had a moderate positive correlation with IOP, but was non\u2010significant (\n \u03c1<\/jats:italic>\n \u2009=\u20090.373,\n p<\/jats:italic>\n \u2009=\u20090.073); IL\u20101\u03b2 had a moderate negative correlation with IOP but was also non\u2010significant (\n \u03c1<\/jats:italic>\n \u2009=\u2009\u22120.344,\n p<\/jats:italic>\n \u2009=\u20090.100). All other pro\u2010inflammatory cytokines had only mild correlation with IOPs (|\n \u03c1<\/jats:italic>\n |\u2009<\u20090.229,\n p<\/jats:italic>\n \u2009>\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n There were no significant changes in the investigated pro\u2010inflammatory cytokines with elevated IOP in canine\n ADAMTS10<\/jats:italic>\n \u2010OAG.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vop.13321","type":"journal-article","created":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T01:20:07Z","timestamp":1739496007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pro\u2010Inflammatory Cytokines in Aqueous Humor From\n \n ADAMTS10<\/i>\n <\/scp>\n \u2010Mutant Beagles at an Early Stage of Open\u2010Angle Glaucoma"],"prefix":"10.1111","volume":"29","author":[{"given":"Elisabeth","family":"Collins","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine D.","family":"Harman","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L.","family":"Jacobson","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Mehaffy","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim R.","family":"Love","sequence":"additional","affiliation":[{"name":"KR Love Quantitative Consulting and Collaboration Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8845-0588","authenticated-orcid":false,"given":"Andr\u00e1s M.","family":"Kom\u00e1romy","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-7790","authenticated-orcid":false,"given":"Michala","family":"de Linde Henriksen","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.13143"},{"key":"e_1_2_10_3_1","first-page":"1173","volume-title":"Veterinary Ophthalmology","author":"Plummer C. 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