{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T05:33:28Z","timestamp":1774848808593,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This report describes the clinical, gross, histopathological, immunohistochemical, and therapeutic aspects of a spontaneous melanocytic iris melanoma in a rabbit, representing the first documented case of this condition in the species in the semiarid region of the state of Rio Grande do Norte, Brazil. A 2\u2010year\u2010old, mixed\u2010breed female rabbit was referred to the Wildlife Department presenting an inadequate diet based on food intended for human consumption. According to the anamnesis, the patient had previously received ocular treatment but later developed bleeding in the right eye. On ophthalmic clinical examination, there was active hemorrhage and ocular rupture, leading to an indication for surgical enucleation. A transpalpebral enucleation was performed, and the eyeball was fixed in 10% buffered formalin and submitted for histopathological evaluation. Histopathological analysis revealed a moderately cellular, unencapsulated, and poorly demarcated neoplasm of the iris and ciliary body, composed of spindloid and anaplastic melanocytic cells containing brown granular pigment, with 12 mitotic figures observed in 2.37\u2009mm\n                    <jats:sup>2<\/jats:sup>\n                    . For immunohistochemical evaluation, melanin bleaching was performed, and a melanoma cocktail (HMB\u201045, Melan\u2010A\/MART\u20101, and Tyrosinase) along with the proliferation marker Ki\u201067 was used. The neoplastic cells showed diffuse cytoplasmic immunoreactivity for all melanocytic markers and nuclear labeling for Ki\u201067, with a nuclear proliferation index of 38%, confirming the diagnosis of melanocytic iris melanoma. Excision of the eyeball using the transpalpebral technique was effective in the treatment of this neoplasm, providing a favorable prognosis. This case highlights the importance of considering iris melanoma as a differential diagnosis for ocular lesions in rabbits.\n                  <\/jats:p>","DOI":"10.1111\/vop.70119","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T04:20:51Z","timestamp":1764390051000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Spontaneous Melanocytic Iris Melanoma in a Rabbit (\n                    <i>Oryctolagus cuniculus<\/i>\n                    Linnaeus, 1758)"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5883-9704","authenticated-orcid":false,"given":"Ana Caroline Freitas Caetano","family":"de Sousa","sequence":"first","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6583-0481","authenticated-orcid":false,"given":"Wanderson Lucas Alves","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2739-3310","authenticated-orcid":false,"given":"Ana Caroline Souza","family":"Maia","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0153-8708","authenticated-orcid":false,"given":"Arthur Vin\u00edcius Caetano","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5329-9059","authenticated-orcid":false,"given":"Jo\u00e3o Augusto Rodrigues Alves","family":"Diniz","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalita Evani Silva","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Histopathological Diagnosis Department VERUM Diagn\u00f3stico, Animal Pathology  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0370-4447","authenticated-orcid":false,"given":"Radan Elvis Matias","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6269-0823","authenticated-orcid":false,"given":"Moacir Franco","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5907-3798","authenticated-orcid":false,"given":"Juliana Fortes Vilarinho","family":"Braga","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed  Bom Jesus Piau\u00ed Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jael Soares","family":"Batista","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0859-3528","authenticated-orcid":false,"given":"Carlos Iber\u00ea Alves","family":"Freitas","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Tumors in Domestic Animals","author":"Goldschmidt M. H.","year":"2017"},{"key":"e_1_2_9_3_1","volume-title":"Withrow and Macewen's Small Animal Clinical Oncology","author":"Friedrichs K. R.","year":"2020"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2015.04.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5505"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.22\u20100181"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.18\u20100081"},{"issue":"3","key":"e_1_2_9_8_1","first-page":"107","article-title":"Four Cases of Melanoma in Rabbits","volume":"23","author":"Yasuda Y.","year":"2014","journal-title":"Journal of Animal Clinical Medicine"},{"key":"e_1_2_9_9_1","volume-title":"Manual de T\u00e9cnicas Para Histologia: Normal e Patol\u00f3gica","author":"Behmer O. A.","year":"2003"},{"issue":"12","key":"e_1_2_9_10_1","first-page":"1143","article-title":"A Comparison of Melanin Bleaching and Azure Blue Counterstaining in the Immunohistochemical Diagnosis of Malignant Melanoma","volume":"12","author":"Kligora C. J.","year":"1999","journal-title":"Modern Pathology"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985820973460"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10070471"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4081\/vsd.2011.3414"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44\u20105\u2010579"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1359426"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1076\/0271-3683(200009)2131-RFT740"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering8120225"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.21037\/aes\u201021\u201030"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2007.03.011"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvex.2016.07.005"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810369898"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810388947"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.22456\/1679\u20109216.84464"},{"key":"e_1_2_9_24_1","volume-title":"Tumors in Domestic Animals","author":"Dubielzig R. R.","year":"2017"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2019.04.008"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70119","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vop.70119","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T04:03:22Z","timestamp":1774843402000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.70119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,28]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/vop.70119"],"URL":"https:\/\/doi.org\/10.1111\/vop.70119","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,28]]},"assertion":[{"value":"2025-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70119"}}