{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:10:47Z","timestamp":1780510247832,"version":"3.54.1"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n \n To confirm and characterize\n Thelazia callipaeda<\/jats:italic>\n infection in a dog using morphological and molecular tools, analyze its phylogenetic relationship with isolates from other regions, and evaluate the clinical and therapeutic outcomes.\n <\/jats:p>\n <\/jats:sec>\n \n Animals Studied<\/jats:title>\n An 8\u2010year\u2010old male mixed\u2010breed dog with a 2\u2010week history of unilateral mucopurulent discharge, epiphora, conjunctivitis, and corneal ulceration in the right eye.<\/jats:p>\n <\/jats:sec>\n \n Procedures<\/jats:title>\n \n Ophthalmic examination revealed multiple thread\u2010like worms in the affected eye. Worms were preserved in 70% ethanol, and genomic DNA was extracted for molecular analysis. A 689 base pair fragment of the mitochondrial cytochrome c oxidase subunit 1 (\n cox1<\/jats:italic>\n ) gene was amplified via polymerase chain reaction, sequenced, and analyzed using Basic Local Alignment Search Tool and Molecular Evolutionary Genetics Analysis (Maximum Likelihood method, 1000 bootstrap replicates) software. Therapeutic management included mechanical removal, systemic ivermectin, and supportive topical therapy, with follow\u2010ups until recovery.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The dog exhibited ocular irritation, lacrimation, and conjunctival hyperemia. Morphological features confirmed\n T. callipaeda<\/jats:italic>\n (distinct buccal capsule, cuticular striations, and encysted larvae in the anterior end of the female worm). The\n cox1<\/jats:italic>\n gene sequence showed 99.4% similarity with isolates from Slovakia and China, with nucleotide substitutions at positions 298 (Cysteine\u2192Threonine), 339 (Alanine\u2192Glycine), 342 (Threonine\u2192Cysteine), and 348 (Glycine\u2192Alanine). Phylogenetic analysis clustered the isolate with European and Asian lineages.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n This report describes the first molecularly confirmed report of\n T. callipaeda<\/jats:italic>\n infection from India, emphasizing the need for increased awareness and regional surveillance of this emerging zoonotic parasite.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vop.70157","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:32:32Z","timestamp":1772994752000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["First Molecular Confirmation of Canine Ocular Thelaziosis Caused by\n \n Thelazia callipaeda<\/i>\n <\/scp>\n From India"],"prefix":"10.1111","volume":"29","author":[{"given":"Alayda N.","family":"Marak","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0515-918X","authenticated-orcid":false,"given":"Suvendu Kumar","family":"Behera","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jashima","family":"Debbarma","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Lawmsanga","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Radiology, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Lalrinkima","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. E.","family":"Chethan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smriti","family":"Lal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry Central Agricultural University Aizawl Mizoram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhishmadev","family":"Sahu","sequence":"additional","affiliation":[{"name":"Office of Deputy Director Veterinary Services Surguja Ambikapur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.05.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.04.013"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12284"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3009.240679"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vprsr.2021.100651"},{"issue":"1","key":"e_1_2_10_7_1","first-page":"86","article-title":"Canine Ocular Thelaziosis in Kerala","volume":"25","author":"Lakshmanan B.","year":"2011","journal-title":"Journal of Veterinary Parasitology"},{"issue":"12","key":"e_1_2_10_8_1","first-page":"62","article-title":"A Case Report of Ocular Thelaziosis in a Dog From Assam","volume":"92","author":"Kakati P.","year":"2015","journal-title":"Indian Veterinary Journal"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-023-01597-4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"issue":"3","key":"e_1_2_10_11_1","first-page":"261","article-title":"The First Comprehensive Study on Isolation and Genetic Characterization of Canine Parvoviruses From Dogs in Mizoram, India Reveals the Emergence of CPV\u20102c","volume":"25","author":"Chakraborty P.","year":"2024","journal-title":"Iranian Journal of Veterinary Research"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.104378"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1404.071205"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1179\/2047773214Y.0000000153"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01335"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0255-0857(21)02478-6"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-4964-y"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70157","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vop.70157","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vop.70157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T03:58:11Z","timestamp":1774843091000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vop.70157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/vop.70157"],"URL":"https:\/\/doi.org\/10.1111\/vop.70157","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70157"}}